The effectiveness of chitosan fruit coating to delay the qualitative and nutraceutical traits of three strawberry cultivars, namely "Candonga", "Jonica" and "Sabrina", as well as the effects of chitosan on antioxidant enzymes were evaluated. The fruits were coated with 1% and 2% chitosan solution and stored at 2 °C for nine days. Samples were taken every three days. Physico-chemical (weight los...

Pericarp browning occurs widely in postharvest pomegranate fruit, but little is known about its mechanism. In this study, ‘Baiyushizi’, a browning-sensitive cultivar, was used to investigate the physiological and genetic changes during pericarp browning. The index (BI) increased greatly after 3 d storage at room temperature, and, consequently, fruit appearance became unattractive 6 d. increasin...

Hydrogen sulfide (H2S) has proved to be a multifunctional signaling molecule in plants and animals. Here, we investigated the role of H2S in the decay of fresh-cut pears (Pyrus pyrifolia). H2S gas released by sodium hydrosulfide (NaHS) prolonged the shelf life of fresh-cut pear slices in a dose-dependent manner. Moreover, H2S maintained higher levels of reducing sugar and soluble protein in pea...

High Quality Antibodies, FISH probes and Kits.Join Memeber Online Order TutorialA Simple Method for Assaying Total Protein, Polyphenol Oxidase and. Several assay procedures have been described for PPO and POD from lychee. PPO.Polyphenoloxidase Activity. The enzyme polyphenoloxidase or tyrosiuase, studying its. Zyme preparation and the general assay procedure.Polyphenol Oxidase Assay. The latter...

Zhang X., Shao X. (2015): Characterisation of polyphenol oxidase and peroxidase and the role in browning of loquat fruit. Czech J. Food Sci., 33: 109–117. Polyphenol oxidase (PPO) and peroxidase (POD) were extracted from a new loquat fruit cultivar (Ninghaibai) and characterised using reliable spectrophotometric methods. In both cases, the optimum pH for PPO was 4.5 and 5 for POD, and their opt...

a completely randomized design (crd) with three replications was conducted at greenhouse of urmia university in 2013 to study the effect of different methyl jasmonate concentrations (0, 0.1 and 0.5 mm) on the activity of enzymes polyphenol oxidase, phenylalanine ammonia lyase, catalase, ascorbate peroxidase, guaiacol peroxidase and total protein content in basil. activity of the enzymes and tot...

A crude enzyme extract from Dioscorea esculenta var. fasiculata tissue subjected to ion exchange chromatography on DEAE-Sephadex A-50 column. This procedure resolved the extract into two main protein peaks one of which eluted through the column relatively unbound while the other protein peak which remained bound to the column was eluted with 1.0 M NaCl. Both protein peaks contained polyphenol o...

Polyphenol oxidase(PPO) (E.C. number 1.10.3.1) has ubiquitous distribution in almost all living organism. Whereas, peroxidase(POD) (E.C. number 1.11.1) act as hormone regulation and defense mechanism in plants. Keeping in pace with their present-day industrial application, efforts have been made to evaluate the activity of these two enzymes (PPO and POD) using pepper pericarp (Capsicum annuum L...

An endophytic bacterium, Bacillus thuringiensis GS1, was isolated from bracken (Pteridium aquilinum) and found to have maximal production of chitinase (4.3 units/ml) at 5 days after culture. This study investigated the ability of B. thuringiensis GS1 to induce resistance to Rhizoctonia solani KACC 40111 (RS) in cucumber plants. Chitinase activity was greatest in RS-treated plants at 4 days. bet...

Wheat is the third largest grown crop after maize and rice worldwide. Integrated use of chemical biofertilizers have potential to improve yield quality due their growth-promoting attributes. Therefore, present study planned evaluate effectiveness endophytic (Paenibacillus sp. strain (ZE11), Bacillus subtilis (ZE15) megaterium (ZE32)) rhizobacterial strains (Bacillus (ZR2) (ZR3) (ZR19)), solely ...