The release kinetics for a variety of proteins of a wide range of molecular mass, hydrodynamic radii, and isoelectric points through a nanofiber hydrogel scaffold consisting of designer self-assembling peptides were studied by using single-molecule fluorescence correlation spectroscopy (FCS). In contrast to classical diffusion experiments, the single-molecule approach allowed for the direct det...

Uncontrolled bleeding from traumatic wounds is a major factor in deaths resulting from military conflict, accidents, disasters and crime. Self-assembling peptide nanofibers have shown superior hemostatic activity, and herein, we elucidate their mechanism by visualizing the formation of nanofiber-based clots that aggregate blood components with a similar morphology to fibrin-based clots. Further...

Title of Document: ACCELERATED SELF-ASSEMBLY OF PEPTIDE-BASED NANOFIBERS USING NANOMECHANICAL STIMULUS Jonathan P. Chang, Master of Science, 2010 Directed By: Assistant Professor/Advisor, Joonil Seog, Department of Materials Science and Engineering One-dimensional nanostructures are ideal building blocks for functional nanoscale assembly. Peptide-based nanofibers have great potential for buildi...

UNLABELLED To speed up the process of central nervous system (CNS) recovery after injury, the need for real-time measurement of axon regeneration in vivo is essential to assess the extent of injury, as well as the optimal timing and delivery of therapeutics and rehabilitation. It was necessary to develop a chronic animal model with an in vivo measurement technique to provide a real-time monitor...

Cutaneous wound repair regenerates skin integrity, but a chronic failure to heal results in compromised tissue function and increased morbidity. To address this, we have used an integrated approach, using nanobiotechnology to augment the rate of wound reepithelialization by combining self-assembling peptide (SAP) nanofiber scaffold and Epidermal Growth Factor (EGF). This SAP bioscaffold was tes...

Controlling the cellular microenvironment is thought to be critical for the successful application of biomaterials for regenerative medicine strategies. Self-assembling peptides are proving to be a promising platform for a variety of regenerative medicine applications. Specifically, RADA16-I self-assembling peptides have been successfully used for 3D cell culture, accelerated wound healing, and...

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Self-Assembling Peptide EAK16 and RADA16 Nanofiber Scaffold HydrogelFabrizio Gelain*Fabrizio GelainInstitute for Stem-cell Biology, Regenerative Medicine Innovative Therapies, IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, 71013, ItalyCenter Nanomedicine Tissue Engineeri...

objective: in this study we introduced an rgd-containing peptide of collagen iv origin that possesses potent cell adhesion and proliferation properties. this peptide was immobilized on a nanofibrous polycaprolactone/gelatin scaffold after which we analyzed human bone marrow-derived mesenchymal stem cells (hbmscs) adhesion and proliferation on this peptide-modified scaffold. methods: nanofibrous...

We demonstrate ultrastrong and flexible hydrogels by self-assembling chitin nanofiber in the presence of gelatin methacryloyl. We tune the mechanical properties of the hydrogel with chitin nanofiber content and show proof-of-concept applications in engineering vascular tissue.