continuous and excessive use of organophosphorus compounds has led to thecontamination of water and soil ecosystems. the degradation of organophosphorusinsecticides, chlorpyrifos, chlorpyrifos- methyl, cyanophos and malathion in mineral saltsmedia were studied. the effect of additional biofertilizers, singly or combined withorganic amendments, on chlorpyrifos and cyanophos degrading activity in...

Azospirillum species are plant growth-promotive bacteria whose beneficial effects have been postulated to be partially due to production of phytohormones, including gibberellins (GAs). In this work, Azospirillum brasilense strain Cd and Azospirillum lipoferum strain USA 5b promoted sheath elongation growth of two single gene GA-deficient dwarf rice (Oryza sativa) mutants, dy and dx, when the in...

; Azospirillum spp. are endophytic bacteria with beneficial effects on cereals — effects partially attributed to gibberellin production by the microorganisms. Azospirillum lipoferum and Azospirillum brasilense inoculated to rice dy mutant reversed dwarfism in seedlings incubated with [17,17-H2]GA20 with formation of [17,17H2]GA1, showing the in vivo capacity to perform the 3 -hydroxylation. Whe...

A lepidopteran toxin gene, cryIC (pSB607) from entomopathogenic Bacillus thuringiensis subsp. aizawai was introduced into nitrogen-fixing Azospirillum lipoferum by transformation. Regeneration of spheroplasts was achieved at 99% with 39% frequency of regeneration. Transformants were screened on NB kanamycin with ampicillin plates and 4 transformants were selected after ten generations. SDS-PAGE...

Flagellation of a nonswimming variant of the mixed flagellated bacterium Azospirillum lipoferum 4B was characterized by electron microscopy, and polyclonal antibodies were raised against polar and lateral flagellins. The variant cells lacked a polar flagellum due to a defect in flagellin synthesis and constitutively expressed lateral flagella. The variant cells were able to respond to condition...

In this study, the effects of deficit water (DW) (normal=control, ½ reduced and 3/4 reduced) some beneficial rhizobacteria (Azospirillum lipoferum, Bacillus megaterium) microalgae (Chlorella saccharophilia) on growth physiological parameters fenugreek (Trigonella foenum-graecum L.) were investigated. The experiment was carried out in a fully controlled climate cabinet with 3 replications factor...

Azospirillum sp. are bacteria beneficial to inoculated plants, and the effect is partially attributed to gibberel lin production. Deutero-labelled GA9 was fed to dy mutant rice-dwarf seedlings inoculated with A. lipoferum or A. brasilense and/or treated with Prohexadione-Ca, an inhibitor of late steps in gibberellin biosynthesis. According to the shoot growth response, the mutant showed the abi...

Three starvation regimes (a deficient culture medium, a saline buffer solution and distilled water) were evaluated for their possible effect on cell surface characteristics of Azospirillum lipoferum 1842 related to the initial adsorption of the bacterium to surfaces. The bacteria survived for 7 days in all media although they did not multiply. Upon transfer from a rich growth medium (nutrient a...