BACKGROUND Babesia spp. are tick-borne protozoan hemoparasites and the second most common blood-borne parasites of mammals, in particular domestic animals. We used the Clustal Multiple Alignment program and 18S rRNA gene sequences of 22 Babesia species from GenBank to develop a PCR that could detect a wide variety of Babesia spp. in a single reaction. The pan-Babesia FRET-qPCR we developed reli...

A study on the presence of Babesia in humans was performed in Puerto Berr o (Latitude 6.50deg. Longitude: -74.38deg. River: Magdalena. Area: 74.410km , Colombia-South America). Indirect immunofluorescence, thin and thick blood smears were used to study 194 individuals. Patients were grouped according to their risk-factors for Babesia infection: (group 1) individuals with fever, chills, sweating...

Serum and DNA from blood samples collected from Vietnamese yellow cattle (n=101) and cattle imported from Thailand (n=54) at a Vietnamese slaughter house were screened for Babesia bovis and Babesia bigemina infections by enzyme-linked immunosorbent assay (ELISA) and PCR. The positive rates determined by ELISA (B. bovis and B. bigemina) or PCR (B. bigemina) in the Vietnamese cattle were signific...

Hemoprotozoan infections often cause serious production losses in livestock. In the present study, we conducted a PCR-based survey of Babesia bovis, Babesia bigemina, Theileria annulata, Theileria orientalis, Trypanosoma evansi and Trypanosoma theileri, using 423 DNA samples extracted from blood samples of cattle (n=202), water buffaloes (n=43), sheep (n=51) and goats (n=127) bred in the Hue an...

Babesia bovis and Cowdria ruminantium were identified for the first time in cattle on Unguja Island, Zanzibar. B bovis is common and widespread, although clinical disease had not been diagnosed previously. The vector of heartwater, Amblyomma variegatum, is found throughout Unguja but C ruminantium appears to be more localised in distribution than B bovis.

Transfection has been a valuable technique for elucidating gene function in many pathogens. While transient transfection of Babesia spp. has been reported previously, stable integration of exogenous genes in Babesia has proven difficult. In this study, a plasmid was designed to target integration of a gfp-bsd gene into the Babesia bovis ef-1alpha locus. Babesia bovis-infected erythrocytes of th...

BACKGROUND There is a little or no data available on the natural Babesia bovis (B. bovis) infection in water buffaloes (Bubalus bubalis) comparing to the available one for cattle. This study was conducted to investigate the natural B. bovis infection in water buffaloes in comparison to crossbred cattle under field conditions in Egypt. METHODS A total of 35 buffaloes and cattle were clinically...

A COMPARATIVE STUDY OF AN ELISA TEST AND AN INDIRECT IMMUNOFLUORESCENCE TEST FOR SEROLOGICAL DIAGNOSIS OF BABESIA BOVIS INFECTION. Detection of antibodies to Babesia bovis in cattle is essential for the understanding of the epidemiology of babesiosis and this study was concerned with comparing the indirect fluorescent antibody with the ELISA. Both assays gave rise to 100% sensitivity whilst the...

b a ckground: there is a little or no data available on the natural babesia bovis ( b . bovis ) infection in water buffaloes( buba l u s bubalis ) comparing to the available one for cattle. this study was conducted to investigate the natural b. bovis infection in water buffaloes in comparison to crossbred cattle under field conditions in egypt. m e t hods: a total of 35 buffaloes and cattle wer...

Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. Babesia parasites have a complex life cycle including asexual replication in the mammalian host and sexual reproduction in the tick vector. Novel control strategies aimed at limiting transmission of the parasite are needed, but transmission blocking vaccine candidates remain undefined. Express...