OBJECTIVES Bactericidal activity depends on antibiotic-bacteria couples, resistance phenotype and theoretically on protein binding. This work explores the influence of protein binding on the bactericidal activity of two antibiotics, daptomycin versus vancomycin, that exhibit, respectively, different C(max) (56 versus 25.5 mg/L), protein binding (91.7% versus 36.9%) and thus theoretical free-dru...

The basis of the bactericidal activity of human lysozyme against Streptococcus sanguis was studied. Experiments were designed to evaluate the role of lysozyme muramidase activity in its bactericidal potency. Inactivation of the muramidase activity of lysozyme was achieved by reduction of essential disulfides with dithiothreitol (DTT) or by incubation with the chitin oligosaccharides chitotriose...

The initial bactericidal activity of quinolones against Streptococcus pneumoniae at the concentration equivalent to their respective peak serum concentration (C(max)) and free drug fraction of C(max) (fC(max)) were investigated. The bactericidal activity of sitafloxacin (STFX), levofloxacin (LVFX), moxifloxacin (MFLX), and garenoxacin (GRNX) were compared by determining the actual killing of ba...

A new method of assessing the bactericidal activity of quinolones and other antibacterial agents, known as the bactericidal index or BI, is introduced and discussed. The BI represents total bacterial kill over a drug concentration range limited to levels achievable in vivo. This method allows the bactericidal activity of drugs to be readily compared. Calculation of BI produces a single value pe...

propolis has traditionally been used in curing infections and healing wounds and burns. current researches have shown that propolis has antibacterial, antifungal and antiviral actions however, the pharmacological activity of propolis is highly variable depending on its geographic origin. there have been few studies on the effects of iranian propolis on the oral microorganisms. in this in-vitro ...

Group IIA secreted phospholipase A2 (sPLA2) is known to display potent Gram-positive bactericidal activity in vitro and in vivo. We have analyzed the bactericidal activity of the full set of recombinant murine and human groups I, II, V, X, and XII sPLA2s on Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli. The rank order potency among human sPLA2s against Gram-positive bacter...

The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to B. pertussis (infected as adults, occupational exposure, immunized with a...

Group IIA secreted phospholipase A(2) (sPLA2) is known to display potent Gram-positive bactericidal activity in vitro and in vivo. We have analyzed the bactericidal activity of the full set of recombinant murine and human groups I, II, V, X, and XII sPLA2s on Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli. The rank order potency among human sPLA2s against Gram-positive bact...

Cefotaxime (HR 756) was compared with cefazolin and cephaloridine with regard to the rate of bacterial killing and to the influence on the bactericidal activity of human serum and polyrnorphonuclear leukocytes. At concentrations equal to 1-2 x MIC cefotaxime showed a more rapid and complete bactericidal activity on E. coil K 12 than cephaloridine and cefazolin. In presence of the latters in fac...