With a large and growing demand in the market, rapeseed (Brassica napus subsp. napus.) is crop with relatively high requirements for macronutrient fertilization. The aim of experiment was to study effect mineral fertilization different doses nitrogen phosphorus on accumulation microelements vegetative organs grains oilseed rape plants.The carried out long-term experimental fields SCDA Livada. T...

Long-term yield performance and yield stability of common cropping systems needs to be simultaneously assessed at various fertility regimes. Based on a consecutive 19-year field trial, including eight fertilization treatments with different combinations of nitrogen (N), phosphorus (P), potassium (K) and recycled manure (RM), the effects of fertilization and crop rotation on corn (Zea mays L.) y...

Quantification of dynamics of soil organic carbon (SOC) pools under the influence of long-term fertilization is essential for predicting carbon (C) sequestration. We combined soil chemical fractionation with stable C isotope analyses to investigate the C dynamics of the various SOC pools after 25 years of fertilization. Five types of soil samples (0-20, 20-40 cm) including the initial level (CK...

In vitro fertilization is becoming a pressing issue in contemporary societies. The article considers it starting from the Polish debate but takes up its broader anthropological and ethical aspects. Dealing with the latter, it also employs some acute remarks from “Dignitas Personae.” Finally it considers an approach to in vitro against the background of “European Convention on Bioethics.” In its...

Following fertilization in mammals, paternal genomic 5-methyl-2'-deoxycytidine (5 mC) content is thought to decrease via oxidation to 5-hydroxymethyl-2'-deoxycytidine (5 hmC). This reciprocal model of demethylation and hydroxymethylation is inferred from indirect, non-quantitative methods. We here report direct quantification of genomic 5 mC and 5 hmC in mouse embryos by small scale liquid chro...

Preimplantation genetic diagnosis (PGD) was performed in two couples to avoid chromosomally unbalanced progeny in a family in which a brother and a sister carry an identical maternally inherited balanced translocation t(3;11)(q27.3;q24.3). Embryos were biopsied 3 days after fertilization and blastomeres were analysed by fluorescent in-situ hybridization (FISH). Embryos were classified as unbala...