The aim of this study was to evaluate the effect of semen collection method (artificial vagina compared to electroejaculation), season in which the semen was collected (breeding season compared to non-breeding season), freezing extender (Biladyl(®), Andromed(®) and skim milk based extender) and pre-treatment procedure (washing compared to non-washing) on post-thaw semen quality in buck. Ejacula...

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Cryopreservation of semen from the Caspian brown trout (Salmo trutta caspius) and effects of extender type, freezing and thawing rates on fertilization ability were studied. After assessment of semen quality, one part of semen was diluted with three parts of different extender and decanted into 0.5ml straws. Freezing was carried out at two freezing levels, 1.5cm and 2cm above surface of liquid ...

The aims of this study were to compare glycerol (G) at customary concentrations and ethylene glycol (EG) as cryoprotectants for stallion semen in a skimmed milk (SM) extender, to test different EG concentrations and to compare the results of manual and computerized analysis with the hypoosmotic swelling (HOS) test. Ejaculates from two stallions were collected over 3 weeks (6 ejaculates per stal...

Due to the recent outbreak of avian influenza, transportation of frozen canine semen with egg yolk has been sharply restricted. Thus, there is urgent need to develop a novel egg yolk-free extender for freezing canine spermatozoa. In the present study, the effect of using skim milk/glucose (SG)-based extender without egg yolk on the motility and fertilizing capacity of canine spermatozoa frozen-...

There are various advantages, such as the preservation of genetic resources, an improvement of the reproduction performance in summer, long-distance transportation of semen and the possibility of progeny test, in the cryopreservation technique of boar semen. However, cryopreserved boar spermatozoa have not been routinely available to swine artificial insemination (AI) due to the lower conceptio...

Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10 spermato...

Effect of Cryopreservation Protocol on Post-Thaw Characteristics of Stallion Spermatozoa. (August 2009) Jose L. Salazar, B.S., Texas A&M University Chair of Advisory Committee: Dr. Dickson D. Varner Three ejaculates from each of eight stallions were initially centrifuged in INRA 96 extender and spermatozoal pellets were resuspended in a milk/egg yolk-based freezing extender or an egg yolk-based...

The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected ejaculates, only semen samples with motility of more than 70% and sperm concentration higher than 3×10 sperm/ml were used for cryopreservation. The selected semen samples were then pooled and dilu...

Fertility of frozen-thawed bull sperm is reduced by cryopreservation. Freezing-thawing procedures can result in as much as a sevenfold fertility decrease. Sperm mortality and loss of motility do not fully explain the reduced fertility of cryopreserved semen; they may be partially explained by the loss of sperm surface proteins, which are necessary for fertilization. We have previously identifie...