BACKGROUND The enzymatic production of biodiesel through alcoholysis of triglycerides has become more attractive because it shows potential in overcoming the drawbacks of chemical processes. In this study, we investigate the production of biodiesel from crude, non-edible Jatropha oil and methanol to characterize Burkholderia cepacia lipase immobilized in an n-butyl-substituted hydrophobic silic...

Oil from brebra (Millettia ferruginea) is the potential raw material for biodiesel production. The refining of crude oil requires a series of purification steps to produce quality biodiesel. The objective of the study was to produce refined oil from brebra seed for biodiesel production. Crude oil extraction was carried out using mechanical press and solvent extraction methods. Impurities of the...

A basic criterion in the selection of microalgae suitable as source of oil for biodiesel should be their actual capacity to produce lipids or, more properly, the fatty acid yield. Performance assessment of 10 preselected microalgae under both batch and continuous culture points to the latter approach as the most adequate for evaluating fatty acid productivity. Differences were patent in continu...

Pichia pastoris CBS 2612 behavior under air pressures of 1, 3 and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-, 1.2-, and 1.5-fo...

Biodiesel, a renewable fuel produced by transesterification of animal fats and vegetable oils, generates about 10% (v/v) of crude glycerol as a core byproduct. The high volume of this non bio-degradable glycerol is becoming of a great environmental and economical concern due to its worldwide ever-growing surplus. Herein we report a high production of 2,3-butanediol (2,3-BD) from pure and biodie...

Increasing demand for plant oil for food, feed, and fuel production has led to food-fuel competition, higher plant lipid cost, and more need for agricultural land. On the other hand, the growing global production of biodiesel has increased the production of glycerol as a by-product. Efficient utilization of this by-product can reduce biodiesel production costs. We engineered Yarrowia lipolytica...

The production of lipids by oleaginous yeast and fungi becomes more important because these lipids can be used for biodiesel production. To understand the process of lipid production better, we developed a model for growth, lipid production and lipid turnover in submerged batch fermentation. This model describes three subsequent phases: exponential growth when both a C-source and an N-source ar...

Renewable energy from lipid removed microalgal residues (LRμARs) serves as a promising tool for sustainable development of the microalgal biodiesel industry. Hence, in this study, LRμAR from Ettlia sp. was characterized for its physico-biochemical parameters, and applied to various pretreatment to increase the biodegradability and used in batch experiments for the production of volatile fatty a...

Biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. Biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (FAMEs) and fatty acid ethyl esters (FAEEs), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (TAGs). TAGs are...

Biodiesel is an alternative diesel fuel consisting of the alkyl monoesters of fatty acids from vegetable oils or animal fats. Currently, most biodiesel is made from soybean oil, methanol, and an alkaline catalyst. However, there are large amounts of fats and oils that are unsuitable for human consumption that could be converted to biodiesel at lower cost. The problem with processing these waste...