Abstract Bean common mosaic virus (BCMV) is one of the most widespread and damaging viruses cultivated legumes in world. In addition to serious yield reduction germplasm decline, BCMV infection also makes more vulnerable other pathogens. Early diagnosis particularly important limiting its spread. Recombinase polymerase amplification (RPA) a novel isothermic technology. The whole reaction can be...

Xenotransplantation is considered to be a solution for the human donor shortage. However, there is a potential risk of transmitting animal infections from the transplanted organ. The known transmissibility and clinical significance of human cytomegalovirus (HCMV) infection after allotransplantation led us to evaluate whether baboon cytomegalovirus (BCMV) transmission could occur after a baboon-...

A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed to simultaneously detect bean common mosaic virus (BCMV), bean common mosaic necrotic virus (BCMNV), and bean golden yellow mosaic virus (BGYMV) from common bean leaves dried with silica gel using a single total nucleic acid extraction cetyl trimethyl ammonium bromide (CTAB) method. A mixture of five specif...

The most effective control of Bean common mosaic virus (BCMV) and necrosis (BCMNV) is achieved by using the seeds resistant cultivars. During conventional breeding, resistance against BCMV BCMNV in bean can be developed pyramiding strain-nonspecific dominant I gene with strain-specific recessive (bc-) genes for long-term control. In this study, a total 58 genotypes involving registered green dr...

Bu çalışma Isparta ve Antalya illerinden toplanan börülce tohum örneklerinde Fasulye adi mozayik virüsü (Bean common mosaic virus: BCMV) Börülce hafif benek (Cowpea mild mottle CpMMV)’nün belirlenmesi amacı ile 2016-2017 yıllarında yürütülmüştür. Tohum örnekleri BCMV CpMMV için Double Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) yöntemi analiz edilmiştir. Elde edilen sonuçlara göre ör...

Antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the presence seed transmissibility of bean common mosaic virus-blackeye cowpea (BCMV-BICM) in farm- retained lots obtained from 46 locations, including markets farms major growing areas Ashanti Brong Ahafo regions Ghana. In growout tests, virus symp...

Bean common mosaic virus from the genus Potyvirus has a wide range of hosts and very negative impact on cultivated crops Phaseolus. The risk viral infection economically important increases even if carriers are related plant species growing agroecological interfaces. Such have emerged as new for BCMV, usually harboring novel genetic variants virus. A variant BCMV was isolated symptomatic crownv...

abstract in 2004 and 2005 growing seasons, 260 bean leaf samples with mosaic, vein-banding, and leaf-rolling symptoms were collected from bean fields of fars, kohgiluye va boyer-ahmad, isfahan and tehran provinces. elisa test (das-elisa and indirect-elisa), using bcmv antiserum, confirmed infection of 110 samples to bcmv. a selected isolate of bcmv from fars province was inoculated on three bea...