We present the initial results of a cytometer that uses an array of extruded high-performance dielectrophoretic traps to capture, hold, and selectively release multiple single bioparticles. We have fabricated small arrays of traps and demonstrated single-bead and single-cell trapping, electrically switched bead release from single traps, fluorescence microscopy of trapped viable single cells, a...

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead id...

In this letter, we report a simple microfluidic device that integrates the capture of bacterial cells using a microscale bead array and the rapid electrical lysis for release of intracellular materials. We study the retention of Escherichia coli cells with different concentrations in this type of bead array and the optimal electrical parameters for the electroporative release of intracellular p...

Inflammatory processes are known to be involved at least in the early phase of complex regional pain syndrome type 1 (CRPS1). Blister fluid obtained from the involved extremities displayed increased amounts of proinflammatory cytokines IL-6 and TNFalpha compared with the noninvolved extremities. The aim of this paper is to investigate the involvement of mediators by measurement of several other...

BACKGROUND We measured the expression of microRNA (miRNA) in non-small cell lung cancer (NSCLC) tissues using the Luminex xMAP bead-based suspension array. We discuss the feasibility of employing this method to detect miRNA in NSCLC and explore its value as a high-throughput miRNA array. METHODS We performed the methodological analysis of xMAP with oligoribonucleic acid references. We detecte...

B-Mode, linear array ultrasonography was used to evaluate the site of semen deposition in live cows. The insemination sheath was modified to hold a brass bead that was deposited at the simulated site of semen deposition. The position of the bead was identified by ultrasonography and the bead was recovered by pulling a nylon line that was attached to the bead. An ultrasound technician identified...

This paper investigates the effects of process parameters on weld bead width of austenitic stainless steel SS-310 in tungsten inert gas welding. The four parameters namely welding current, type of gas, gas flow rate and included angle of weld plates during butt joint were varied at three levels. The DOE approach was used to design experimental conditions. Orthogonal array L9 was used for carryi...

We report a new approach to designing an artificial nose based on high-density optical arrays that directly incorporate a number of structural and operational features of the olfactory system. The arrays are comprised of thousands of microsphere (bead) sensors, each belonging to a discrete class, randomly dispersed across the face of an etched optical imaging fiber. Beads are recognized and cla...