to produce embryogenic callus and somatic embryos, tendril explants were collected and cultured in ms medium supplemented with various concentrations of hormones. after one month most of explants produced callus. the highest percent of embryogenic callus obtained in ms medium supplemented with 4.5 µm bap and 1µm 2,4-d. production of somatic embryos was depended on genotype and medium. yaghouti ...

abstract to produce embryogenic callus and somatic embryo, whole flower explants were collected at two sampling stages of i, iii. to produce embryogenic callus and somatic embryo, ms medium supplemented with 5 and 10µm 2,4-d and 2µm bap were used. for embryo differentiation, ms medium with 0.5mg/l iba, ms medium without any plant growth regulators, ms medium with 2mg/l iba and 0.2mg/l bap, ms m...

protection and the prevention of illegal departures national grape germplasm, as one of the most important assets of the country, requires identification of the commercial and native cultivars and registered them in the international centers. the present project was conducted in order to registration the most important grape cultivars of iran based on morphological and physiological traits in t...

to produce embryonic callus in four grapevine cultivars i.e. yaghouti, bidane sefid, shahroodi and flame seedless, ovary explants were collected at flower developmental stages iii and v and cultured in ms medium supplemented with various concentrations of 2,4-d and bap. for differentiation of somatic embryos, ms medium was supplemented with various concentrations of iaa and bap. results showed ...

in this research, the effect of different concentrations of chitosan (with low molecular weight) on in vitro proliferation of grape cultivar, ’bidaneh ghermez‘, was evaluated. 30 days after establishment, elongated shoots were cultured on the half strength murashige and skoog medium supplemented with 0.5 mg/l bap, 0.1 mg/l iba and different chitosan concentrations (0, 10, 20, 40 and 80 mg/l). t...

frost in early spring is one of the limiting factors in the production of grape. chemicals offer useful approach to reduce low temperature-induced damages. different concentrations of salicylic acid including: 0 (control), 0.5, 1 and 2 mm were sprayed twice on 10 year-old ‘bidaneh sefid’ grape vines at full bloom stage and changes in leaf soluble carbohydrates, proline and protein levels were m...