A detailed study of the qualitative and quantitative composition of bile acids in human fetal gallbladder bile is described. Bile was collected during early gestation (weeks 16-19) and analyzed by gas chromatography and mass spectrometry, fast atom bombardment ionization mass spectrometry, and high performance liquid chromatography. Bile acids were separated into different conjugate groups by c...

The main bile acids in human bile are cholic, chenodeoxycholic, and deoxycholic with a mean ratio of 1.1:1.0:0-6 (Sjovall, 1960). Cholic and chenodeoxycholic acid are primary bile acids synthesized from cholesterol in the liver (Bergstrom, Danielsson, and Samuelsson, 1960). These bile acids are not interconvertible. The secondary bile acids, deoxycholic and lithocholic, are products formed by t...

CONTEXT Besides their role in intestinal resorption of lipids, bile acids are regarded as endocrine and metabolic signaling molecules. The detailed profile of bile acid species in peripheral blood after an oral lipid tolerance test (OLTT) is unknown. OBJECTIVE We quantified the regulation of 18 bile acids after OLTT in healthy individuals. MATERIAL AND METHODS 100 volunteers were characteri...

The main bile acids in human bile are cholic, chenodeoxycholic, and deoxycholic acids,l which are present as conjugates with taurine or glycine. Cholic and chenodeoxycholic acids are the “primary” bile acids formed from cholesterol in the liver, whereas deoxycholic acid is a product of microbial dehydroxylation of cholic acid in the intestine during the enterohepatic circulation of bile (1). In...

The effect of side chain conjugation on 7-dehydroxylation of bile acids has been investigated. C24-bile acids and their glycine and taurine conjugates and keto bile acids were incubated with pure strains of Eubacterium sp. VPI 12708. Bile acids of the 5 alpha- or 5 beta-series with a free terminal carboxyl group and a 3 alpha, 7 alpha-dihydroxy system were very effectively 7 alpha-dehydroxylate...

The principles governing the in vitro solubility of the common natural conjugated and unconjugated bile acids and salts in relation to pH, micelle formation, and Ca2+ concentration are considered from a theoretical standpoint and then correlated first with experimental observations on model systems and second with the formation of precipitates containing bile acids in health and disease. In vit...

This review makes an attempt to combine the insights gained into the biochemistry and physiology of bile acids with the elegant supramolecular systems designed from them. Bile acids are cholesterol-derived facial amphiphiles responsible for the solubilization of cholesterol and fat through mixed micelle formation with phospholipids. An intriguing aspect of bile acids is that their chemical stru...

Bile acid homeostasis is tightly maintained through interactions between the liver, intestine, and kidney. During cholestasis, the liver is incapable of properly clearing bile acids from the circulation, and alternative excretory pathways are utilized. In obstructive cholestasis, urinary elimination is often increased, and this pathway is further enhanced after bile duct ligation in mice that a...

Biliary secretion in health and disease is reviewed. The powerful techniques of molecular biology have enabled cloning of the transporters involved in biliary secretion and the enterohepatic circulation of bile acids. This, in turn has permitted elucidation of their function as well as their regulation by nuclear receptors. Bile acid secretion is required for efficient lipid absorption, and bil...

The effect of microbiological deconjugation of bile acids on total bile acid and neutral sterol fecal excretion by adult male rats has been studied. A screening method utilizing mice allowed selection of a Clostridium perfringens type A strain, which accelerated cholesterol catabolism in mice. When this species of bacteria was associated with germfree rats, the fecal bile acids were excreted as...