The aim of this study was to develop and validate a simple, sensitive, precise and cost-effective one-level agar diffusion (5+1) bioassay for estimation of potency and bioactivity of Levofloxacin in pharmaceutical preparation which has not yet been reported in any pharmacopoeia. Among 16 microbial strains, Bacillus pumilus ATCC-14884 was selected as the most significant strain against Levofloxa...

Abstract Background and Aims: The Cydia pomonella granulovirus (CpGV) is a baculovirus and very effective biological control agent against apple pest, codling moth, Cydia pomonella L. especially for healthy organic apple production and protection.  Not only, screening of CpGV isolates via biochemical variation, but also their biological variation is practically necessary.  Howeve...

Some degree of error is inevitable in multi-agent bioassays regardless of design or measurement technology. Estimation error can be reduced post facto by exploiting the matrix partial ordering of the bioassay survival estimates. The standard method for this is order-restricted regression (ORR). If the joint action of the bioassay agents admits a tolerance-based interpretation, additional struct...

A new bioassay method for measurement of angiotensin II was clinically applied to determine the plasma renin activity (PRA) in normal subjects and hypertensive patients. Angiotensin II was extracted by a modified procedure reported by Boucher et al. Levels of angiotensin II extracted from 17 blood samples were independently determined by our new bioassay method and the rat pressor assay. The ne...

The objective of this study was to compare a bioassay with a liquid chromatography–fluorescence–mass spectrometry method for detection of enrofloxacin (ENRO) in incurred eggs. The bioassay developed by our laboratories involves an agar diffusion microbiological method using Klebsiella pneumoniae as an indicator organism. Results demonstrate that both methods are capable of detecting incurred fl...

A new selective high-performance liquid chromatography (HPLC) method with UV detection for the determination of the investigational triazole voriconazole in human plasma by using acetonitrile precipitation followed by reverse-phase HPLC on a C(18) column was compared with a simple agar well diffusion bioassay method with Candida kefyr ATCC 46764 as the assay organism. Pooled plasma was used to ...