Affinity tags such as polyhistidine greatly facilitate recombinant protein production. The solubility of integral membrane proteins is maintained by the formation of protein-detergent complexes (PDCs), with detergent present at concentration above its critical micelle concentration (CMC). Removal of the affinity tag necessitates inclusion of an engineered protease cleavage site. A commonly util...

Integral membrane proteins (IMPs) are crucial biological components, mediating the transfer of material and information between cells and their environment. Many IMPs have proven to be difficult to isolate and study. High-resolution structural information on this class of proteins is limited, largely because of difficulties in generating soluble forms of such proteins that retain native folding...

O well-established application of biotechnology is the use of enzymes in detergents. Enzymes are proteins that act as biochemical catalysts and that increase the rate of specific reactions by several orders of magnitude (usually g10). Cells cannot function without enzymes, but under controlled conditions, enzymes can work outside of the cell. This discovery led to the development of a growing n...

O well-established application of biotechnology is the use of enzymes in detergents. Enzymes are proteins that act as biochemical catalysts and that increase the rate of specific reactions by several orders of magnitude (usually g10). Cells cannot function without enzymes, but under controlled conditions, enzymes can work outside of the cell. This discovery led to the development of a growing n...

commercial detergents, in spite of their various names and marks, are used to remove foreign matter (dirt) from substrate. following removal of dirt from the surface, it is necessary to prevent redeposition, so the detergent must have supending power. in this experimental work, a simple method has been developed to evaluate the cleaning power of commercial detergents. the method, showing the ch...

Lipid rafts are small plasma membrane domains that contain high levels of cholesterol and sphingolipids. Traditional methods for the biochemical isolation of lipid rafts involve the extraction of cells with nonionic detergents followed by the separation of a low-density, detergent-resistant membrane fraction on density gradients. Because of concerns regarding the possible introduction of artifa...

Although detergents are often essential in protocols, they are usually incompatible with further biochemical analysis. There are several methods for detergent removal, but the procedures are complicated or suffer from sample loss. Here, we describe a convenient and rapid method for detergent removal from sialic acid-containing glycosphingolipids (gangliosides) and neutral glycolipids in deterge...

of the detergents used (Evans, 1956), although the sharp cut-off in activity evident lends support to the idea that protein denaturation by anionic detergents involves the formation of protein-detergent micelles (Tanford, 1970). The most effective inhibitor of glucuronidase activity was sodium tridecyl sulphate, whereas sodium pentadecyl sulphate exerted the strongest effects on the sulphatase....