The immunofluorescence-based detection of c-H2AX is a reliable and sensitive method for quantitatively measuring DNA double-strand breaks (DSBs) in irradiated samples. Since H2AX phosphorylation is highly linear with radiation dose, this wellestablished biomarker is in current use in radiation biodosimetry. At the Center for High-Throughput Minimally Invasive Radiation Biodosimetry, we have dev...

© 2016 Genome Integrity | Published by Wolters Kluwer Medknow Open Access Russia, Singapore, Sri Lanka, Thailand, UK, Ukraine, Uruguay, and Vietnam. The CRP established links with the Incident and Emergency Centre, World Health Organization BioDoseNet, Coordination Action “Realizing the European Network of Biodosimetry,” Japanese National Institute of Radiological Science (currently, the Nation...

Medical management of radiation emergencies will require quick and reliable biodosimetric tools for assessment absorbed dose. Dicentric chromosomal assay (Gold standard) has a limitation being time intensive, requires specialised human skill cannot be used triage mass screening. Dose assessments suspected individuals are critical the medical emergencies. For effectively utilizing available reso...

Receiver operating characteristic (ROC) analysis is a fundamental tool used for the evaluation and comparison of diagnostic systems that provides estimates of the combinations of sensitivity and specificity that can be achieved with a given technique. Along with critical considerations of practical limitations, such as throughput and time to availability of results, ROC analyses can be applied ...

Objective(s): Nowadays, ionizing radiation (IR) has a significant contribution to the diagnostic and therapeutic medicine, and following that, health risks to individuals through unexpected exposure is greatly increased. Therefore, biological and molecular technology for estimation of dose (biodosimetry) is taken into consideration. In biodosimetry methods stimulation ...

Consideration of statistical methodology is essential for the application of cytogenetic and other biodosimetry techniques to triage for mass casualty situations. This is because the requirement for speed and accuracy in biodosimetric triage necessarily introduces greater uncertainties than would be acceptable in day-to-day biodosimetry. Additionally, in a large scale accident type situation, i...

The World Health Organization (WHO) held a consultation meeting at WHO Headquarters, Geneva, Switzerland, December 17-18, 2007, to develop the framework for a global biodosimetry network. The WHO network is envisioned to enable dose assessment using multiple methods [cytogenetics, electron paramagnetic resonance (EPR), radionuclide bioassays, etc.]; however, the initial discussion focused on th...