Introduction Perfusion process involves retention of the cells inside the bioreactor while simultaneously removing spent medium and adding fresh medium continuously. The flow rate of addition of fresh and removal of spent medium are generally kept the same (perfusion rate) to maintain the culture volume inside the bioreactor. Cell retention is possible with many devices but the reliability and ...

Bioprocess scale-down simulators are used to investigate the phenomena occurring in industrial scale cultivations. The aim is to simulate the large-scale conditions, characterized by long mixing times introducing local gradients into the bioreactor, in laboratory scale bioreactor. We have expanded the current physiological knowledge about the consequences of such cultivation conditions from wel...

BACKGROUND Biological activated sludge process must be functionally stable to continuously remove contaminants while relying upon the activity of complex microbial communities. However the dynamics of these communities are as yet poorly understood. A macroecology metric used to quantify community dynamic is the taxa-time relationship (TTR). Although the TTR of animal and plant species has been ...

Background: Nowadays, the optimization of the culture conditions in bioreactors is considered as an approach to produce secondary metabolites such as thebaine. Thebaine is the dominant alkaloid in Iranian poppy that is used as a precursor for the synthesis of analgesic compounds. Objective: Optimization of culture conditions of Papaver bracteatum adventitious roots in bioreactor for the scale...

One of the major steps toward the industrialization of the microbial product(s) is to optimize the cultivation conditions at the large-scale bioreactor and successfully control the microbial behavior within a large scale production environment. Statistical Design of Experiment was proven to optimize a vast number of microbial processes to achieve robustness and explore possible interactions...

Background Vaccination has proven itself as the most effective tool to control and prevent the disease and to facilitate the safe trade of live animals. Viral vaccine manufacturing processes present some specific constraints as compared to other biotech products linked to the cell substrate used and to the viral production. Multiple cell lines are used for productions such as VERO, MDCK, MRC5, ...

The impact of bioreactor and sparger scale-up on gas transfer properties was investigated using four geometrically similar bioreactors ranging in size from 15-L to 2500-L total volume with the same sintered metal frit (microporous) sparger design. The mass transfer coefficients for both oxygen and carbon dioxide were measured at each scale at various flow rates. Scale did not directly affect ga...

aerobic digestion was useful for mixed sludge of west ahwaz sewage treatment plant. results showed that after 12 days 38% of tvs reduced. this meets the environmental standards. after 19 days detention time in the bioreactor, cod, vss, tvs, tss, ts, were 40 ,56, 54.5 ,50.4 ,44.7 percent respectively. kd coefficient was,0.173day-1. aim of this bench scale study is determination of aerobic digest...

Miniature parallel bioreactors are becoming increasingly important as tools to facilitate rapid bioprocess design. Once the most promising strain and culture conditions have been identified a suitable scale-up basis needs to be established in order that the cell growth rates and product yields achieved in small scale optimization studies are maintained at larger scales. Recently we have reporte...