Sulfate-rich mine water must be treated before it is released into natural water bodies. We tested ethanol as substrate in bioreactors designed for biological sulfate removal from mine water containing up to 9 g L-1 sulfate, using granular sludge from an industrial waste water treatment plant as inoculum. The pH, redox potential, and sulfate and sulfide concentrations were measured twice a week...

molecular farming, or bio-pharming, has recently received much of attention for production of valuable recombinant proteins, with a few already being marketed. the use of whole plants for synthesis of pharmaceutical proteins offers various advantages in economy, scalability and safety over conventional production systems. gm plants are suitable for the inexpensive production of large amounts of...

Plant bioreactors are attractive expression systems for economic production of pharmaceuticals. Various plant expression systems or platforms have been tested with certain degrees of success over the past years. However, further development and improvement are needed for more effective plant bioreactors. In this review we first summarize recent progress in various plant bioreactor expression sy...

Recent studies have shown that vapor-phase bioreactors containing the fungus Exophiala lecanii-corni can remove nitric oxide (NO) and volatile organic compounds efficiently from waste gas streams. However, when NH4 accumulates in the bioreactor, the NO removal efficiency abruptly drops to 20 %. To achieve high NOx removals in the bioreactors, low NH4 levels must be maintained, or a different ni...

the aim of the present study was to develop a tissue-engineering approach through alginate gel molding to mimic cartilage tissue in a three-dimensional culture system. the perfusion biomimetic bioreactor was designed to mimic natural joint. the shear stresses exerting on the bioreactor chamber were calculated by computational fluid dynamic (cfd). several alginate/bovine chondrocyte constructs w...

Background Single-use bioreactors continue to gain large interest in the biopharmaceutical industry. They are excessively used for mammalian cell cultivations, e.g. production of monoclonal antibodies and vaccines [1]. This is motivated by several advantages of these bioreactors like reduced risk of cross contaminations or shortening lead times [2]. Singleuse bioreactors differ in terms of shap...

Continuous bioreactors are critical unit operations in a wide variety of biotechnological processes. While they can be viewed as chemical reactors, bioreactors offer unique modeling and control challenges due to the complexity of the underlying biochemical reactions and the distributed properties of the cell population. The dynamic behavior of continuous bioreactors can be strongly affected by ...

Rationale and significance. Recombinant therapeutic proteins are frequently produced in mammalian cells cultivated in suspension in large-scale stirred tank bioreactors. There are three major drawbacks to stirred-tank bioreactors. First, they are expensive to purchase and maintain, limiting their availability to some potential users. Second, they have not been adapted to volumetric scales less ...

Experimental bioreactors operated as recirculated closed systems were inoculated with bacterial cultures that utilized methane, propane, and tryptone-yeast extract as aerobic carbon and energy sources and degraded trichloroethylene (TCE). Up to 95% removal of TCE was observed after 5 days of incubation. Uninoculated bioreactors inhibited with 0.5% Formalin and 0.2% sodium azide retained greater...

Introduction In order to maximize cell growth within a compact space and retain cells for easy medium exchange, the iCELLis bioreactors from ATMI LifeSciences contain macro-carriers trapped in a fixed-bed, creating a 3-D matrix within which cells adhere and replicate. These bioreactors also enable precise temperature, pH and dissolved oxygen control which cannot be done in 2-D cultures. The iCE...