The author and his co-workers have found out that, when the diluents including egg yolk for freezing of boar spermatozoa were used, egg yolk agglutinated immediately after the dilution and most of the spermatozoa were enclosed into the agglutinated masses and their motility was drastically suppressed. On the other hand, by using skim milk-diluent widely used for storing liquid boar semen at 15°...

We evaluated the ability of immature pig oocytes (at germinal vesicle stage) to detect differences in the in vitro penetration rates of boar spermatozoa. In Experiment 1, immature and ovulated oocytes (n=303) were exposed to capacitated boar spermatozoa to determine if the penetrability of immature pig oocytes was comparable to that of ovulated oocytes. The percentages of penetrated oocytes and...

It is known that several materials used in semen collection have been found to be detrimental to spermatozoal motility. In this study, examinations for toxic effects of latex and vinyl gloves, used with and without talcum powder on boar spermatozoa, were performed. Ten boars of known fertility with "280%sperm motility were divided into two groups (n = 5 boars each) for in vitro and in vivo stud...

Eriksson, B. 2000. Cryopresewation of boar semen. Studies on sperm viability in vitro and fertility. Doctoral thesis. ISSN 1401-6257. ISBN 91-576-5903-6 The world’s pig population is consistently being upgraded through the international trade of superior genetics. The two major systems that are used for this purpose are the transport of live animals and the export of frozen boar semen. The main...

Artificial insemination in pig offers many advantages in swine production in terms of a better disease control through semen quality control, a diverse male genetic distribution and an easiness of management. It is accepted that in developing countries, AI helps to improve the genetic profile. A number of sows can be inseminated using the same ejac‐ ulate instead of only one from natural mating...

Optimal sperm cryopreservation is a prerequisite for the sustainable commercial application of frozen-thawed boar semen for AI. Three experiments were performed to identify factors influencing variability of postthaw sperm survival among 464 boar ejaculates. Sperm-rich ejaculate fractions were cryopre-served using a standard freezing-thawing procedure for 0.5-mL plastic straws and computer-cont...

Six gilts were artificially inseminated (AI) with extended semen from a boar free of porcine reproductive and respiratory syndrome (PRRS)virus infection. One week later, the same boar was inoculated intranasally with PRRSvirus. Seven days after inoculation,the boar was used to AI an additional five gilts. All II gilts were bred 3 days in a row using freshly collected and extended semen on each ...

This study attempted to clarify the relationship between the levels of 70kDa heat shock protein (HSP70) and semen quality in boars. Semen samples from 29 (13 Duroc, 9 Landrace, and 7 Yorkshire) boars (mean age=25.2+/-2.2 months) were examined. Three to four ejaculates per boar, collected during cool and hot seasons, were evaluated in terms of the sperm concentration, sperm motility, percentage ...

The present paper highlights aspects of the cryopreservation of boar semen, a species with particular large, fractionated ejaculates, and a cumbersome cryotechnology that had prevented its commercial application. With the dramatic increase of use of liquid pig semen for artificial breeding over the past decade, developments on cryopreservation alongside the routine use of stud boar semen for AI...

Boar semen cryopreservation remains a challenge due to the extension of cold shock damage. Thus, many alternatives have emerged to improve the quality of frozen-thawed boar sperm. Although the use of seminal plasma arising from boar sperm-rich fraction (SP-SRF) has shown good efficacy; however, the majority of actual sperm evaluation techniques include a single or dual sperm parameter analysis,...