Bordetella spp. are not normally included when considering the opportunistic bacterial species that are typically involved in respiratory tract infections in individuals with cystic fibrosis (CF). By using a combination of bacterial genotyping and 16S rDNA sequencing, Bordetella spp. were identified in cultures obtained from 43 individuals with CF. Most (n = 23) patients were infected with Bord...

We concur with Al-Murieb and colleagues that pertussis outbreaks are a potential and probably under-recognised problem in aged-care facilities.1 In February 1999, we investigated an outbreak of acute respiratory infection, which had affected over 50% of the residents of a Sydney nursing home. Serological and virological testing pointed to influenza A as the major cause of illness in 19 of 35 co...

The evolution of Bordetella pertussis and Bordetella parapertussis from Bordetella bronchiseptica involved changes in host range and pathogenicity. Recent data suggest that the human-adapted Bordetella modified their interaction with host immune systems to effect these changes and that decreased stimulation of Toll-like receptor 4 (TLR4) by lipid A is central to this. We discuss Bordetella lipi...

background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...

Species of the genus Bordetella associate with various animal hosts, frequently causing respiratory disease. Bordetella pertussis is the primary agent of whooping cough and other Bordetella species can cause similar cough illness. Here, we report four complete genome sequences from isolates of different Bordetella species recovered from human respiratory infections.

Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.

Bordetella pertussis 18323 produces a bvg-regulated 39.1-kDa porin-like protein, OmpQ. OmpQ had 61% similarity to the major porin of B. pertussis and contains conserved regions common to both the neisserial and enteric porin families. The results of Southern blot analysis indicate that strains of Bordetella parapertussis and Bordetella bronchiseptica but not Bordetella avium contain this gene.

1. Lutvik LI. Infections in asplenic patients. En: Mandell GL, Bennett JE, Dolin R, editores. Principles and Practice of Infectious Diseases. 6th ed. Philadelphia: Elsevier Churchill Livingstone; 2005. p. 3524–32. 2. Steinberg MH. Management of sickle cell disease. N Engl J Med. 1999;340:1021–30. 3. Weyant RS, Hollis DG, Weaver RE, Amin MFM, Steigerwalt AG, O’Connor SP, et al. Bordetella holmes...

We report here the complete genome sequences of Bordetella flabilis and Bordetella bronchialis recovered from cultures of individuals with cystic fibrosis (CF), and "Bordetella pseudohinzii" recovered from a CF mouse model.