Inhibin is a gonadal hormone that inhibits the release of follicle stimulating hormone (FSH) from the anterior pituitary gland. The objective of this study was to determine whether active immunization of male and female rats against inhibin rich, steroid-free bovine follicular fluid would increase inhibin antibody titre, onset of female puberty, pregnancy rate, litter size, testis weights, test...

Abstract Phthalates are ubiquitous environmental contaminants and impact reproduction epigenetic mechanisms. The use of diisobutyl phthalate (DIBP) has increased in the last decade. However, studies about DIBP's effects on female reproductive physiology limited. Methylation DNA RNA processes that regulate gene expression, cell biology, functions. How DIBP exposure regulates these mechanisms ova...

In mammals, IGFs are important for the proliferation and steroidogenesis of ovarian cells. Metformin is an insulin sensitizer molecule used for the treatment of the infertility of women with polycystic ovary syndrome. It is, however, unclear whether metformin acts on ovarian cells. Adenosine 5' monophosphate-activated protein kinase (AMPK) is involved in metformin action in various cell types. ...

SUMMARY Concentrations of luteinizing hormone (LH) and follicle stimulating hormone (FSH) were determined in serum of crossbred beef bulls during pubertal development. Serum LH ranged between .5 and 2.0 ng/ml with highest concentrations observed as 12 months of age. Serum FSH varied considerably among individuals but mean concentrations were within the normal range (50 to 100 ng/ml). Neither LH...

Cyclic nucleotides cAMP and cGMP are among the main molecules that control maturation of mammal oocytes. The in vitro simulated physiological oocyte (SPOM) method was used to model accumulation oocyte. In current study, we preincubated oocytes cows (not primed with FSH) modulators: N 6,2’-O-dibutyryladenosine 3’,5’-cyclomonophosphate (dbcAMP) 3-isobutyl-1-methylxanthine (IBMX). use SPOM increas...

The production of activin by Sertoli cells isolated from 21-day-old rats was studied using the mesoderm-inducing activity of activin on Xenopus laevis animal cap explants, immunoprecipitation and Western blotting. Furthermore, the effects of recombinant bovine activin-A on rat Sertoli cell aromatase activity and FSH and androgen receptor gene expression were examined. Animal cap explants from X...

The objective of the study was to examine the effect of lysophosphatidic acid (LPA) on 17β-estradiol (E2) synthesis and follicle stimulating hormone (FSH) action in bovine granulosa cells. We found that granulosa cells in the bovine antral follicle, in addition to the uterus and the CL, are also the site of LPA synthesis and the target for LPA action in the bovine reproductive tract. Our findin...

In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their actions, based on high affinity binding to specific membrane receptors, are quite different. The purpose of this study was to perform a sensitive localization of FSH and LII in secretory granules of gonadot...

Follicle-stimulating hormone (FSH; follitropin) is a pituitary glycoprotein composed of two post-translationally modified subunits, which must properly assemble to be biologically active. FSH has been difficult to purify and to obtain in quantities sufficient for detailed biochemical studies. We have targeted FSH expression to the mammary gland of transgenic mice by using cDNAs encoding the bov...