invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...

this study was conducted to detect the presence ofseja gene among salmonella enteritidis isolates from poultry sources by polymerase chain reaction (pf'r) and evaluate its potential as diagnostic and epidemiological tools. thirty salmonella isolates from poultry sources: broilers. broiler breeders, layers, hatcheries, and poultry abattoirs were investigated. upper and forward primers were ...

The objective of the study was to genotype Pandharpuri buffalo breeds for k-casein type by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A 379-bp k-casein gene fragment was amplified using primers K1 (5’CAC GTC ACC CAC ACC CAC ATT TAT C-3’) and K2 (5’-TAA TTA GCC CAT TTC GCC TTC TCT GT-3’). DNA from 20 Pandharpuri buffaloes were extracted by phenol chloroform me...

introduction butyrivibrio fibrisolvens strains are presently recognized as the major butyrate-producing bacteria found in the rumen and digestive track of many animals and also in the human gut. in this study we reported the development of two dna based techniques, quantitative competitive (qc) pcr and absolute based real-time pcr, for enumerating butyrivibrio fibrisolvens strains. despite the ...

with the thymine residues were isolated and ligated together in a 10-μL reaction mixture (overnight at room temperature) using equal amounts of each fragment. The ligation was terminated by heating to 65°C, and the products were introduced into DH5α E. coli cells. Cells were grown under ampicillin selection and white colonies selected for miniprep isolation. Plasmid DNA was digested with PvuII...

Objective: Identity the genetic aspects and major gene influence on energy balance, milk production, fertility, food safety and consumer are the recent interests of genetic and breeding researchers. Methods: Najdi Cattle is the most prominent breeds in Khuzestan province. To do this plan in Shoushtar Najdi Cattle Station, blood samples were taken from 15 Najdi Cattles. DNA was extracted from wh...

Objective: Identity the genetic aspects and major gene influence on energy balance, milk production, fertility, food safety and consumer are the recent interests of genetic and breeding researchers. Methods: Najdi Cattle is the most prominent breeds in Khuzestan province. To do this plan in Shoushtar Najdi Cattle Station, blood samples were taken from 15 Najdi Cattles. DNA was extracted from wh...

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

an attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(pcr-rflp). blood samples were taken from 50 swamp buffaloes and 50 murrah buffaloes. the dna was extracted by the phenol-chloroform method. the pcr-rflp was performed using beta-lactoglobulin ...