Adult neurogenesis is often studied by labeling new cells with the thymidine analog bromodeoxyuridine (BrdU) and using immunohistochemical methods for their visualization. Using this approach, considerable variability has been reported in the number of new cells produced in the dentate gyrus of adult rodents. We examined whether immunohistochemical methods, including BrdU antibodies from differ...

Immunohistochemical assessment of 5-bromo-2-deoxyuridine (BrdU) in tissue sections is a widely used method to evaluate cell proliferation in vivo. However, this method requires time-consuming preparation of paraffin sections and laborious counting of BrdU-labeled nuclei on multiple sections. Here, we report the development of a rapid and reliable method to quantitate BrdU incorporation in intes...

Chick embryos were explanted at stages ranging from the definitive streak to one-somite and cultured for 19-21 h on thin albumen with or without 5-bromodeoxyuridine (BrdU) (2-12/ig/ml). BrdU primarily inhibited neurulation which was found to be stage dependent. If exposed to BrdU at the definitive streak stage, it resulted in an open brain region; if treatment was delayed until the head-process...

.......................................................................................................5 Introduction................................................................................................6-17 Adult neurogenesis: a controversial history.............................................................7-8 BrdU labeling to track adult neurogenesis.................................

Parkinson's disease (PD) is characterized by motor symptoms with depression. We evaluated the influence of dopaminergic depletion on hippocampal neurogenesis process to explore mechanisms of depression production. Five consecutive days of 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) injection in mice (MPTP-mice) reduced dopaminergic fibers in hippocampal dentate gyrus (DG). MPTP-mice exh...

Labelling of DNA in replicating cells using 5-bromo-2´-deoxyuridine (BrdU) is widely used, however the rapid clearance and metabolisation of BrdU in the living organism is a critical issue. Although the pharmacokinetic of BrdU in experimental animals is empirically approximated, the exact time-curve remains unknown. Here we present novel method for estimation of the BrdU content in the blood se...

introduction: this study was designed to investigate the effects of granulocyte colony-stimulating factor (g-csf) administration in rats for 6 weeks after traumatic brain injury (tbi). methods: adult male wistar rats (n = 30) were injured with controlled cortical impact device and divided into four groups. the treatment groups (n = 10 each) were injected subcutaneously with recombinant human g-...

Twelve patients with ependymomas received a 30- to 60-minute intravenous infusion of bromodeoxyuridine (BrdU), 150 to 200 mg/m2 at surgery, to label tumor cells in the DNA synthesis phase. Labeled cells were detected in excised tumor specimens by indirect immunoperoxidase staining using anti-BrdU monoclonal antibody as the first antibody. The BrdU labeling index (LI, defined as the percentage o...