Genome-wide identification of RNAs associated with RNA-binding proteins is crucial for deciphering posttranscriptional regulatory systems. PUMILIO is a member of the evolutionary conserved Puf-family of RNA-binding proteins that repress gene expression posttranscriptionally. We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we ...

During spermatogenesis, germ cells initially expand exponentially through mitoses. A majority of these cells are then eliminated through p53-mediated apoptosis to maintain germline homeostasis. However, the activity of p53 must be precisely modulated, especially suppressed in postmitotic spermatogenic cells, to guarantee robustness of spermatogenesis. Currently, how the suppression is achieved ...

Many stem cell populations interact with stromal cells via signaling pathways, and understanding these interactions is key for understanding stem cell biology. In Drosophila, germline stem cell (GSC) maintenance requires regulation of several genes, including dpp, piwi, pumilio, and bam. GSCs also maintain continuous contact with cap cells that probably secrete the signaling ligands necessary f...

Abiotic factors have a strong infl uence on where annual Bromus species are found. At the large regional scale, temperature and precipitation extremes determine the boundaries of Bromus occurrence. At the more local scale, soil characteristics and climate infl uence distribution, cover, and performance. In hot, dry, summerrainfall-dominated deserts (Sonoran, Chihuahuan), little or no Bromus is ...

Abundance of invasive plants is often attributed to their ability ot outcompete native species. We compared resource acquisition and allocation of the invasive annual grass Bromus madritensis subsp. rubens with that of two native Mojave Desert annuals, Vulpia octoflora and Descurainia pinnata, in a glasshouse experiment. Each species was grown in monoculture at two densities and two levels of N...

Poison frogs in the anuran family Dendrobatidae use bright colors on their bodies to advertise toxicity. The species Dendrobates pumilio Schmidt 1858, the strawberry poison frog, shows extreme polymorphism in color and pattern in Panama. It is known that females of D. pumilio preferentially choose mates of their own color morph. Nevertheless, potential predators must clearly see and recognize a...

The genes belonging to Pumilio gene family of the fission yeast Schizosaccharomyces pombe (S. pombe) were compared with genome information of the several fungi and their functions have been inferred using public-access databases disclosed on the internet websites. The Pumilio-family genes are conserved from yeast to man and have been considered to suppress the expression of other genes by bindi...

Carcinops pumilio (Erichson) were collected using two trapping methods: a black light pitfall trap and a mesh-bottomed trap placed on poultry manure. C. pumilio collected with black lights and subsequently starved had a significantly higher dispersal rate during days 1-3 than fully fed groups. When densities of < 500 Caloglyphus berlesei (Michael) (Acarina: Acaridae) per 50 C. pumilio were prov...

Light and scanning electron micrographs of Opisthorchis viverrini, Haplorchis taichui, H. pumilio, A Phaneropsolus bonnei, and Prosthodendrium molenkampi eggs were studied. Under light microscopy. O. viverrini eggs had rough eggshells and prominent shoulders. H. taichui, H. pumilio were similar in shape and had smooth eggshells and prominent shoulders. H. pumilio eggs were bigger than H. taichu...

Why some species exhibit remarkable variation among populations while closely related species are relatively uniform remains unclear. The strawberry dart-poison frog (Dendrobates pumillo) exhibits spectacular color and pattern polmorphism among populations in the Bocas del Toro archipelago of Panama. In contrast, two other sympatric species of dart-poison frog, Phyllobates lugubris and Minyobat...