A microagglutination test was compared with a standard tube agglutination test for the detection of brucella antibodies. Advantages of the microagglutination test were that it required less time to perform, had a shorter incubation period, and used less antigen.

BACKGROUND AND OBJECTIVES Brucellosis is a zoonotic disease and it's still endemic in Iran. There are some reports regarding brucellosis infection in family members sharing same risk factors and remain unrecognized. However, few studies on the importance of family screening are available. We aimed to screen household members of index cases with acute brucellosis for detecting additional unrecog...

ABSTRACT: The present study described the prevalence and factors associated with seropositivity for anti-Brucella spp. antibodies in working equids semiarid regionofnortheastern Brazil. Blood samples were collected from 322 (76 horses, 155 mules 91 donkeys). Rose Bengal plate test (RBPT) was used as a screening method. Samples that reactive RBPT tested confirmation using 2-mercaptoethanol (2-ME...

OBJECTIVE The primary objective of the study was to determine the prevalence of brucellosis and the antimicrobial susceptibility pattern of local Brucellae isolates in the Infectious Diseases Hospital, Kuwait. SUBJECTS AND METHODS A single serum sample was collected from each of 1,836 patients of different nationalities from January 2000 to December 2001. Any patient with a provisional diagno...

TO THE EDITOR—The recent article by Guven et al [1], titled “Neurobrucellosis: Clinical and Diagnostic Features,” sheds light on many important yet vexing issues in the clinical presentation, diagnosis, andmanagementofarelativelycommon zoonotic infection. The relevance of discussing this issue is understated by the lack of consensus in the diagnosis and management of neurobrucellosis, in combin...

In order to verify the frequency of human brucellosis among a TB suspected population, a study was conducted on the basis of sero-diagnosis of a total of 229 blood samples from TB suspected patients. Serological tests carried out were slide and semi-quantitative agglutination tests. Of the tested samples, 44 (19.21%) were SAT positive and of these, 28 (12.23%) were positive for the semiquantita...

The few cytogenetic studies performed in both humans and animals exposed in vivo or in vitro to dioxin reported conflicting data with or without chromosome damage. Therefore the aim of this work was to fulfill the genotoxicity of dioxin and its effect on immune response of sheep vaccinated with Brucella melitensis Rev.1 vaccine. A total number of twenty female goats were divided into four group...

Aim: In the diagnosis of brucellosis, production microorganisms in blood or bone marrow culture is considered gold standard. But it not always possible to produce microorganism. For this reason, serological tests are used diagnose brucellosis. Rose Bengal test (RB), Standard Tube Agglutination (STA), Coombs Test (CT) and 2-Mercaptoethanol (2-ME) common methods. Immunocapture-agglutination (ICA)...

BACKGROUND Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic valu...