Objective(s):Brucellosis, especially caused by Brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. The commonly used live attenuated vaccine in ovine brucellosis prophylaxis is B. melitensis Rev1. But due to different problems caused by the administration of this vaccine, a protective subunit vaccine against B. me...

Background: To prevent the losses caused by brucellosis, development of diagnostic tools for differentiating vaccinated cattle from infected one is priority in eradication disease. The present study was undertaken differential identification Brucella abortus vaccine strain S19 EryC PCR. Methods: In this study, 1145 clinical samples unvaccinated and buffaloes (200 blood samples, 710 sera, 190 va...

Brucella bacterium causes Brucellosis, an infectious disease spreading from animals to human. Brucella lumazine synthase (BLS) is a highly immunogenic protein with adjuvant properties, which has been introduced as an effective protein carrier for vaccine development. This protein also plays a significant role in inducing immune system. This study aimed to clone, express, and purify the BLS gene...

Brucellosis is an infectious disease with high impact on innate immune responses which is induced partly by its DNA. In the present study the potential differences of wild type and patients isolates versus attenuated vaccine strains in terms of cytokines, ROS and NO induction on murine splenocytes and peritoneal macrophages were investigated. This panel varied in base composition and included D...

objective(s):brucellosis, especially caused by brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. the commonly used live attenuated vaccine in ovine brucellosis prophylaxis is b. melitensis rev1. but due to different problems caused by the administration of this vaccine, a protective subunit vaccine against b. me...

Brucellosis is a zoonotic and economically significant animal disease worldwide. The most frequently used vaccine to avoid brucellosis in small ruminants the Rev1 conjunctival Brucella melitensis vaccine. aim of this study was investigate effects B.melitensis Revl on total protein, albumin, globulin levels, protein profiles oxidant/antioxidant status sheep. Ten sheep were as material for purpos...

BACKGROUND Brucellosis is a zoonosis that occurs worldwide. The disease has been completely eradicated in livestock in Sweden in 1994, and all cases of confirmed human brucellosis are imported into Sweden from other countries. However, due to an increase in the number of refugees and asylum seekers from the middle-east to Sweden, there is a need to improve the current diagnostic methodology for...

Brucellosis is an infection that affects mammals’ cells caused by a species from the genus Brucella, gram-negative, immobile, facultative, intracellular, and microaerophilic coccobacillus. In humans, pathogen are Brucella melitensis, B. abortus, suis canis. We presenting case of 62-year-old man with confirmed brucellosis his consequent cardiovascular, cutaneous, gastrointestinal, hepatic, osteo...

brucellosis is an infectious disease with high impact on innate immune responses which is induced partly by its dna. in the present study the potential differences of wild type and patients isolates versus attenuated vaccine strains in terms of cytokines, ros and no induction on murine splenocytes and peritoneal macrophages were investigated. this panel varied in base composition and included d...