This paper describes the DNA damage by hydrogen peroxide in HL-60 cells and the detection of 8-hydroxyguanine produced. The number of cells was at least about 2 x 10(7)/sample. Treatment of HL-60 cells with 0.4 mmol/L hydrogen peroxide for 24 h would result in DNA damage. The DNA samples were isolated, then qualitatively and quantitatively analyzed before hydrolysis. The results of experiments ...

The mono(μ-oxo) dicopper cores present in the pores of Cu-ZSM-5 are active for the partial oxidation of methane to methanol. However, copper on the external surface reduces the ratio of active, selective sites to unselective sites. More efficient catalysts are obtained by controlling the copper deposition during synthesis. Herein, the external exchange sites of ZSM-5 samples were passivated by ...

We read carefully the very interesting article by Jones et al. (1), which appears in the latest issue of the Journal. To our surprise, we realized that, during the determination of opiates in blood, although the described derivatization procedure involves BSTFA, the authors use, for their qualitative and quantitative analysis, mass fragments that correspond to their pentafluoroderivatives and t...

When we subjected 11-dehydrothromboxane B2 (11-DTXB2), a metabolite of arachidonic acid, to standard chemical derivatization procedures we obtained a mixture of several products. Separation of the components was carried out by gas chromatography and their identification was accomplished through the study of their mass spectra, which are presented here. Anomalous behaviors include methylation of...

Purpose: To develop a simple yet sensitive, reliable and robust method for qualitative determination of cocaine and a series of its tropane metabolites in biological samples (blood, urine, etc.). The method should be optimized for routine forensic expertise in analytical toxicology. Material/Methods: Gas chromatography coupled with mass spectrometry (GC-MS) has been used as the method for analy...

Allamanda violacea is an ornamental plant of the genus Allamanda belongs to family Apocynaceae. Different flower extracts of the plant have been reported to possess anti-hyperlipidemic, anti-hyperglycemic and anti-oxidant activities. Present study deals with the identification of phytochemical constituents of the petroleum ether (pet ether) and chloroform extract by GC-MS analysis, and the cons...