Vegetative propagation of Persian walnut (Juglans regia L.) is more difficult than many other fruit trees. Temperature, relative humidity, water status the scion and rootstock, nutrient uptake, time grafting, physiological condition type all can affect grafting success. An experiment was conducted to evaluate effects post-budding irrigation frequency (3, 7 10-days), budding date (14 June, 5 26 ...

The prevailing hypothesis of HIV budding posits that the viral Gag protein drives budding, and that the Gag p6 peptide plays an essential role by recruiting host-cell budding factors to sites of HIV assembly. HIV also expresses a second Gag protein, p160 Gag-Pol, which lacks p6 and fails to bud from cells, consistent with the prevailing hypothesis of HIV budding. However, we show here that the ...

We have used correlated scanning EM (SEM) and multiphoton fluorescence microscopy to visualize budding of virus-like particles (VLPs) of Rous sarcoma virus (RSV) and HIV type 1 (HIV-1). When the Gag structural protein was expressed alone as a GFP fusion, most budding particles appeared morphologically aberrant, but normal assembly could be rescued by coexpression of untagged Gag protein. Imagin...