Different semen assays were employed to determine the post -thaw fitness of buffalo and Sahiwal bull spermatozoa. Semen (two ejaculates) was collected from buffalo and Sahiwal bulls (six each) once a week for three consecutive weeks. Semen was diluted in lactose-fructose-egg yolk glycerol extender, equilibrated at 4 ac for 4 hr and frozen in liquid nitrogen vapors. A highly significant differen...

The authors describe a serological survey on the prevalence of infectious bovine rhinotracheitis (IBR) among cattle and buffalo (Bubalus bubalis) in three southern states of India. A local isolate of bovine herpesvirus 1 (BHV-1) from an outbreak of the respiratory form of IBR was used as a source of virus antigen in avidin-biotin enzyme-linked immunosorbent assay (ELISA). The overall prevalence...

The study had two objectives, namely, to estimate the andrological disorders leading to disposal of Karan Fries (KF), Sahiwal cattle and Murrah buffalo bulls and to study the effect of various factors (species/breeds, season of birth and period of birth) on male reproductive parameters. Records on occurrence of subfertility problems and disposal pattern of bulls maintained at the National Dairy...

To study the influence of season on oxidative status of buffalo semen and their association with semen characteristics and seminal plasma proteins, ejaculates were collected twice a week in winter, summer and rainy seasons from six adult Bhadawari buffalo bulls. The neat semen was analyzed for semen characteristics immediately after collection and oxidative status viz. lipid peroxidation (LPO),...

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

The in sacco protein and protein-free dry matter rumen degradability of three feeds, concentrate, alfalfa hay and maize silage, was determined simultaneously in three animal species: four Mediterranean buffalo (Bubalus bubalis) bulls, four Friesian bulls and four Delle Langhe rams, which received four diets differing in forage:concentrate ratio (1 5 87.5:12.5; 0.75 2 5 75.0:25.0; 3 5 62.5:37.5;...

The dimensions of spermatozoa from different levels of the reproductive tract were measured in 6 buffalo-bulls castrated under local anesthesia ; the same measurements were taken in the semen of 6 breeding buffalo-bulls. During transit from caput to cauda epididymis the sperm showed a significant increase in the length of head and main piece. However, a significant decrease was observed in midd...

East coast fever is a disease of cattle caused by Theileria parva and is transmitted by the tick Rhipicephalus appendiculatus. Buffalos are a natural reservoir of the parasite. The objectives of this study were to determine the T. parva infection in buffalo and cattle field samples, to identify genotypes of T. parva infecting cattle and buffalo and finally to investigate the presence of buffalo...

Interspecific hybrid embryos are useful models for the study of maternal-fetal interactions, transmission pattern of species-specific markers and parental contributions to growth and developmental potential of pre-attachment embryos. In an attempt to investigate the possibility of producing hybrid embryos of domestic cattle (Bos taurus) and water buffalo (Bubalus bubalis), cattle oocytes were e...