Abstract The enzyme urate oxidase (UOX) is used as a drug for preventing and treatment of hyperuricemia. This study deals with the statistical optimization lactose inducible fermentation production soluble recombinant Aspergillus flavus UOX. 10 independent variables were investigated by Plackett–Burman design (PBD), most significant factors further optimized central composite (CCD). PBD results...

Pectinases (EC 3.2.1.15) are a class of enzymes that catalyze the depolymerization or de-esterification reactions degrade pectic substances. In present study, we have isolated Aspergillus niger strain from soil samples, in India and evaluated pectinase production. The highest producing A. was further optimized with various agricultural wastes. Plackett-Burman design (PBD) Central composite ‘(CC...

The conversion of methane into liquid biofuels using methane-consuming bacteria, known as methanotrophs, contributes to sustainable development, it mitigates the problem climate change caused by greenhouse gases and aids in producing cleaner renewable energy. In present research, an efficient methanotroph, Methylosarcina sp. LC-4, was studied a prospective organism for biodiesel production meth...

Pectinases find wide application in different industrial applications, e.g., textile, food, tea, paper and pulp, vegetable oil extractions, saccharification of agricultural remains, fermented drinks. Three fungal strains were screened for pectinase production. Results indicated that all are capable producing a enzyme. Penicillium chrysogenum MF318506 showed the highest activity (0.214 U/ml) on ...

NW-G01, produced by Streptomyces alboflavus 313, is a novel cyclic hexapeptide antibiotic with many potential applications, including antimicrobial activity and antitumor agents. This study developed a system for optimizing medium components in order to enhance NW-G01 production. In this study, Plackett-Burman design (PBD) was used to find the key ingredients of medium components, and then resp...

As a potential intermediary feedstock, succinic acid takes an important place in bulk chemical productions. For the first time, a method combining Plackett-Burman design (PBD), steepest ascent method (SA), and Box-Behnken design (BBD) was developed to optimize Actinobacillus succinogenes ATCC 55618 fermentation medium. First, glucose, yeast extract, and MgCO(3) were identified to be key medium ...

a solid state fermentation method was used to utilize wheat straw as substrates for citric acid production by using aspergillus niger atcc 9142. the plackett burman design (pbd) of experiments was used to test the relative importance of the variables affecting production such as moisture content, age of spore, inoculum size, initial ph of substrate, methanol concentration, incubation temperatur...

Background A glycolipopeptide biosurfactant produced by Pseudomonas aeruginosa strain IKW1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/L. The compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. Method In the present study, Plackett...

In this study a new method of preconcentration, separation and efficient measuring of so-called hollow fiber solid / liquid phase microextraction has been introduced for the determination of trace amounts of lorazepam (LRZ) in water samples, urine and hair using differential pulse voltammetry. To this end, a hybrid adsorbent material was used that involved ionic liquid assisted magnetic multi-w...

In this study, the optimization of medium components used for production lipase enzyme from Cryptococcus albidus D24 was performed using Plackett-Burman statistical design method (PBD), and most important nutrients affecting strain were determined as first step. According to PBD, highest activity (19.34 U/ml/min) obtained with including Tween 80 (X2) 2.5% (v/v), (g/L) Peptone (X4) 8.0, Yeast Ex...