conclusions this research study proved the presence of viable probiotic lab microflora in the ewe milk with enhanced health benefits. the 2 selected lactobacillus strains could be exploited in dairy or pharmaceutical industry in future. objectives the probiotic efficacy of two lactobacillus strains namely lactobacillus pentosus lp05 and l. brevis lb32 was evaluated. they were previously isolate...

PURPOSE To predict Caco-2 permeability is a valuable target for pharmaceutical research. Most of the Caco-2 prediction models are based on commercial or special software which limited their practical value. This study represents the relationship between Caco-2 permeability and molecular descriptors totally based on open source software. METHODS The Caco-2 prediction model was constructed base...

Coenzyme Q10 (CoQ10) is a key component of the mitochondrial electron transfer chain and is one of the most important antioxidants. We previously found that glycoprotein prosaposin (Psap) binds CoQ10 in human cells. Although Psap is expressed in the intestines, its role in the gastrointestinal tract is not clear. To elucidate the role of Psap in the intestines, we established Psap knockdown (KD...

The absorption characteristics of temocapril were investigated using Caco-2 cells, and the esterases expressed in Caco-2 cells were identified. Temocapril was almost completely hydrolyzed to temocaprilat during transport across Caco-2 cells. Hydrolysis experiments of temocapril in Caco-2 cell 9000g supernatant (S9) and brush-border membrane vesicles showed that temocapril was mainly hydrolyzed ...

Ma, Thomas Y., Michel A. Boivin, Dongmei Ye, Ali Pedram, and Hamid M. Said. Mechanism of TNFmodulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression. Am J Physiol Gastrointest Liver Physiol 288: G422–G430, 2005; doi:10.1152/ajpgi.00412.2004.—TNFplays a central role in the intestinal inflammation of various inflammatory disorders inc...

Several in vitro assays have been developed to evaluate the gastrointestinal absorption of compounds. Our aim was to compare 3 of these methods: 1) the bio-mimetic artificial membrane permeability assay (BAMPA) method, which offers a high-throughput, noncellular approach to the measurement of passive transport; 2) the traditional Caco-2 cell assay, the use of which as a high-throughput tool is ...

in this paper, a new continuous ant colony optimization (caco) algorithm is proposed for optimal reservoir operation. the paper presents a new method of determining and setting a complete set of control parameters for any given problem, saving the user from a tedious trial and error based approach to determine them. the paper also proposes an elitist strategy for caco algorithm where best solut...

Ma, Thomas Y., Michel A. Boivin, Dongmei Ye, Ali Pedram, and Hamid M. Said. Mechanism of TNFmodulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression. Am J Physiol Gastrointest Liver Physiol 288: G422–G430, 2005; doi:10.1152/ajpgi.00412.2004.—TNFplays a central role in the intestinal inflammation of various inflammatory disorders inc...

Ma, Thomas Y., Gary K. Iwamoto, Neil T. Hoa, Vimesh Akotia, Ali Pedram, Michel A. Boivin, and Hamid M. Said. TNFinduced increase in intestinal epithelial tight junction permeability requires NFB activation. Am J Physiol Gastrointest Liver Physiol 286: G367–G376, 2004; 10.1152/ajpgi.00173.2003.—Crohn’s disease (CD) patients have an abnormal increase in intestinal epithelial permeability. The def...