POLI F., Tosi B., DALL'OLIO G. & BRUNI A. 1992. Production of thiophenes in calli and suspension cultures of Tagetes patula L. as influenced by light/dark succession. — Phyton (Horn, Austria) 31 (2): 257-262, 3 figures. English with German summary. HPTLC-densitometry was used to evaluate thiophene accumulation in leaf calli and cell suspension of Tagetes patula. Calli obtained from 7-8 weeks ol...

Еру Optimization of the regeneration potential in vitro for maize (Zea mays L.) hybrids with participation perspective inbreds is an actual task biotechnological maintenance this important crop breeding process.
 The purpose research was investigation effect auxin phytohormone — indolylbutyric acid (IBA) on capacity hybrids. work to determine IBA concentrations 0.1 and 0.2 mg / L total fre...

the present study was aimed at the development of a somoclonal variant and isozyme marker for phyllanthus amarus schum & thonn using inter-nodal segments and the enzyme peroxidase. maximum callus proliferation was obtained on murashige and skoog’s medium supplemented (ms) with 1.0 mg/l of 2,4-dichlorophenoxyacetic acid. three weeks-old pale yellowish white semi-friable callus was used for o...

J. curcas has been studied in different countries and some interesting agronomic, pharmacological and industrial properties have been reported. More recently, it has been considered an important alternative source for biofuel production. The objective of this study was to establish a long-term method for the maintenance of calli and cell suspension cultures of the local species J. curcas and J....

Concentration of mineral elements in calli of six sunflower inbred lines have been determined. Calli were induced from cotyledons of seven days old seedlings grown under sterile conditions. The differences between inbred lines in concentration of N, P, K, Mg, Fe and Mn were significant, whereas differences in the concentration of Ca, Na, Cu and Zn were insignificant. Average concentrations of m...

The cDNA encoding the 326 amino acids of a Class I basic chitinase gene from Leucaena leucocephala de Wit (KB3, Genbank accession: AAM49597) was cloned under the control of CaMV35S promoter in pCAMBIA 1300 and transferred to Koshihikari. Calli of Koshihikari rice was transformed with agrobacterium with this construct expressing the chitinase and βglucouronidase (GUS). The frequencies of calli 9...

Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together wi...

Different sugarcane varieties (CP 77/400, CP 85/1491 and Punjabi) were checked for its callus induction and regeneration capabilities using different growth regulators and explants (eye bud, upper portion of inner leaf whorls and lower portion of inner leaf whorls). Seven callus induction media i.e. CIM-1 to CIM-6 and control media, five shoot induction media i.e. SIM-1 to SIM-4 and control med...

organogenesis and neoformant plantlet production were studied by culturing the different parts of mature embryo including radicle and proximal and distal parts of cotyledon of mission and kroneiki cultivars. different parts of embryos were cultures in omc medium containing growth regulators, such as indole butyric acid (iba) and 2-isopentenyl adenine (2-ip) or iba and naphthalene acetic acid (n...

Shoot regeneration in calli derived from immature barley embryos is regulated by light conditions during the callus-induction period. Barley cultivars Kanto Nijo-5 (KN5) and K-3 (K3) showed lower efficiency of shoot regeneration in a 16-h photoperiod during callus-induction than those in continuous darkness, whereas shoot regeneration was enhanced in cultures under a 16-h photoperiod in Golden ...