The already differentiated organs in plants have a remarkable capacity to regenerate new individuals under culture conditions. Plant in vitro regeneration practically starts with the induction of a pluripotent cell mass, the callus, from detached organs on auxin-rich callus-inducing medium (CIM), which is generally required for subsequent regeneration of new bodies. Recent studies show that CIM...

Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium clad...

Objective: To develop in vitro callus induction in nodal segments of Decalepis hamiltonii and investigate the antioxidant potential of the callus. Methods: In this study, nodal segments of Decalepis were cultured on Murashige and Skoog (MS) medium supplimented with various concentration of 2,4-D for callus induction and later subcultured on various concentration of NAA and BAP. The antioxidant ...

Introduction Cumin (Cuminum cyminum L.) is a commercial plant which is valued for its aroma and its medicinal and therapeutic properties. Although India has been the primary exporter of cumin seeds and cumin oil in the world. However Turkey and Iran are providing stiff competition now. India, Turkey, Syria, China, the US, Iran, Indonesia, Sudan, Egypt, Morocco, Algeria, and Libya are the leadin...

Eight cultivars Sorghum bicolor (L.) Moench were grown as callus cultures under induced, prolonged water stress (8 weeks), with polyethylene glycol in the medium. Concentrations of soluble carbohydrates and organic acids in callus were measured at the end of the growth period to determine differences in response to prolonged water stress. Sucrose, glucose, fructose, and malate were the predomin...

OBJECTIVES Locked plating constructs may be too stiff to reliably promote secondary bone healing. This study used a novel imaging technique to quantify periosteal callus formation of distal femur fractures stabilized with locking plates. It investigated the effects of cortex-to-plate distance, bridging span, and implant material on periosteal callus formation. DESIGN Retrospective cohort stud...

A methanol extract of Gymnema sylvestre leaf and callus showed anti-diabetic activities through regenerating β-cells. Optimum callus was developed under stress conditions of blue light with 2,4-D (1.5 mg/l) and KN (0.5 mg/l), which induced maximum biomass of green compact callus at 45 days, as determined by growth curve analysis. Leaf and optimum callus extracts contains gymnemic acid, which wa...

Plants develop unorganized cell masses like callus and tumors in response to various biotic and abiotic stimuli. Since the historical discovery that the combination of two growth-promoting hormones, auxin and cytokinin, induces callus from plant explants in vitro, this experimental system has been used extensively in both basic research and horticultural applications. The molecular basis of cal...

Unselected and sodium sulfate tolerant callus cultures of Brassica napus L. cv Westar were grown on media supplemented with mannitol, NaCl, or Na(2)SO(4). In all cases, growth of tolerant callus, measured on a fresh weight or dry weight basis, was greater than that of unselected callus, which was also subject to necrosis on high levels of salt. Tissue water potential became more negative in bot...

embryogenic callus initiation from anther and whole flower explants in four grapevine cultivars including flame seedless, shahroodi, perlette and red sultanina cultured on piv and harst nutrient media was studied. results showed that whole flower explants of ‘flame seedless’ cultured on piv medium had the most potential for callus production. later callus were transferred onto ges1ca medium for...