Candida species are common human fungal pathogens causing a wide range of clinical diseases, ranging from superficial infections to life-threatening systemic disease. Superficial infections include vaginal candidiasis which affects over 75% of women during their lifetime with 5% of women suffering debilitating recurrent infections. Life-threatening systemic Candida disease is the fourth most co...

Candida albicans and C. glabrata are the two most common pathogenic yeasts of humans, yet they are phylogenetically, genetically and phenotypically very different. In this review, we compare and contrast the strategies of C. albicans and C. glabrata to attach to and invade into the host, obtain nutrients and evade the host immune response. Although their strategies share some basic concepts, th...

Candida species are a common cause of nosocomial bloodstream infections. Recent surveillance has shown an increase in the relative proportion of infections caused by Candida glabrata, which has reduced susceptibility to fluconazole. We undertook sentinel surveillance with antifungal susceptibility testing to monitor the trends in the proportions of various Candida species causing invasive disea...

Objective To identify the most frequent Candida species in specimens from patients hospitalized different medical centers of Mexico City, with suspected fungal infection. Methods Specimens were grown on Sabouraud dextrose agar at 28°C for 72 h. In addition, DNA was extracted. Isolates CHROMagar Candida™, 37°C 48 The molecular identification performed by polymerase chain reaction (PCR) using pri...

background recent evidences have revealed that in more than 65% of microbial infections, biofilms have critical roles. the ability of yeasts to form biofilms on surface of medical devices such as urine catheters is an important reason of the capability of these microorganisms to cause human disease. objectives the aim of this study was to isolate and identify yeasts on the surfaces of urine cat...

Precise FKS mutation rates among Candida species are undefined because studies have not systematically screened consecutive, disease-causing isolates. The Sensititre YeastOne (SYO) assay measures echinocandin MICs against Candida with less variability than reference broth microdilution methods. However, clinical breakpoint MICs may overstate caspofungin nonsusceptibility compared to other agent...

Candida species are the fourth most frequent cause of nosocomial bloodstream infections, and 25%-50% occur in critical care units. During an 18-month prospective study period, all patients admitted for > or = 72 hours to the surgical (SICUs) or neonatal intensive care units (NICUs) at each of the participant institutions were followed daily. Among 4,276 patients admitted to the seven SICUs in s...

background and purpose: despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple treatment-related side-effects. the present study was designed to investigate the antifungal properties of different extracts from pistacia atlantica desf. materials and methods: different parts of p. atlantica (i.e., dried ...

Here, we report a method for the transformation of Candida glabrata using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection in the human fungal pathogen Candida glabrata ATCC2001 and related strains. Furthermore, t...

In this study, 1717 fungal clinical isolates causing invasive fungal infections were evaluated against nine antifungal agents using Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution methods. The isolates comprised 1487 Candida spp., 109 Aspergillus spp., 86 non-Candida yeasts (including 52 isolates of Cryptococcus neoformans) and 35 rare moulds obtained during 201...