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Microfilariae of Dirofilaria immitis were recovered from an experimentally infected dog and inoculated intravenously into adult female BALB/c mice. Each mouse received 1 X 10(5) microfilariae. In one experiment mice were sublethally irradiated with gamma radiation 24 h prior to microfilarial inoculation; microfilariae were counted at 11.00 and 23.00 h 7, 14, 21, 28, 35 and 42 days after inocula...

INTRODUCTION Human dirofilariasis is a zoonotic infection caused by the filarial worm, Dirofilaria (Nochtiella) repens, whose primary host is the dog. This infection is on the increase over the past decade in Sri Lanka and the prevalence of canine dirofilariasis in the country is also believed to be high. We present here a study on public awareness of dirofilariasis and the prevalence of this i...

ABSTRACT A canine patient, 13-year-old mixed breed male, 24kg, was seen at a private clinic in the city of Guayaquil, Ecuador. The main reason for consultation evaluation mass right knee and neck that had previously been cytologically diagnosed neoplastic origin by another team veterinarians through fine needle puncture cytology. contacts were asymptomatic. At time consultation, detailed explor...

The present study was conducted to determine the prevalence of canine dirofilariosis in Hatay province, south of Turkey. A total of 269 blood samples were collected from owned dogs in this multi-centre survey between March and July 2006. Blood samples were examined by modified Knott and enzyme-linked immunosorbent assay (ELISA) techniques to detect circulating microfilariae and antigens of Diro...

The aim of this study was to make the first report on canine heartworm disease in the state of Rondônia and confirm its transmission in this state. Blood samples were randomly collected from 727 dogs in the city of Porto Velho. The samples were analyzed to search for microfilariae and circulating antigens, using three different techniques: optical microscopy on thick blood smears stained with G...

Microfilariae obtained from in vitro culture of adult Dirofilaria immitis were inoculated into naive dogs and used to test the in vivo efficacy of the antimicrofilarial drug Dizan. Injection of 33 million microfilariae into a 3-month-old male beagle pup over a 5-day period resulted in a microfilaremia that peaked at 863 microfilariae per ml on day 30. Treatment with Dizan resulted in a rapid cl...

Onchocerciasis is a major cause of blindness worldwide, and much of the blindness is caused by onchocercal chorioretinitis. In an experimental animal model for ocular onchocerciasis, intravitreal injections of 10,000 live Onchocerca volvulus microfilariae isolated from infected humans into the eyes of cynomolgus monkeys (Macaca fascicularis) resulted in patchy, progressive loss of retinal pigme...

The zoonotic parasite Dirofilaria repens has spread toward north in Europe, and cases of autochthonous dirofilariosis caused by D. have emerged the Baltic countries Estonia, Latvia, Lithuania. We conducted a review on emergence humans domestic dogs these three northeastern Europe. Based available literature reports, first finding was made Latvia 2008, followed Lithuania 2010, Estonia 2012. In a...