BMP2, like its Drosophila homologue dpp, is an important signaling molecule for specification of cardiogenic mesoderm in vertebrates. Here, we analyzed the time-course of BMP2-requirement for early heart formation in whole chick embryos and in explants of antero-lateral plate mesoderm. Addition of Noggin to explants isolated at stage 4 and cultured for 24 h resulted in loss of NKX2.5, GATA4, eH...

background and aim: iron overload in patients with thalassemia major is one of the most important complications of this disease and its cardiac complications are the most important causes of death in these patients.mri t2 is the perfect device to assess the amount of iron deposited in the heart of thalassemia patients, but it causes waste of time and also is an expensive diagnostic method. tiss...

We identified hyaluronan synthase-2 (Has2) as a likely source of hyaluronan (HA) during embryonic development, and we used gene targeting to study its function in vivo. Has2(-/-) embryos lack HA, exhibit severe cardiac and vascular abnormalities, and die during midgestation (E9.5-10). Heart explants from Has2(-/-) embryos lack the characteristic transformation of cardiac endothelial cells into ...

An in vitro assay has been developed to investigate tissue interactions regulating myocardial cell specification in birds. Explants from the posterior region of stage XI-XIV blastulas were found to form heart muscle at high frequency with a timing that corresponded to onset of cardiac myocyte differentiation in vivo. Isolation and recombination experiments demonstrated that a signal from the hy...

The Tungkal Composite of liberica coffee rejuvenation program at Jambi Province is urgently needed due to the latest status plants are old and showing diseased attacked. To support rejuvenation, provision seedlings could be done by tissue culture approach through somatic embryogenesis. This research aimed study effect 2,4-D, BA TDZ on callus formation embryo induction from leaf explants. Explan...

Recently, novel H7N9 influenza viruses have caused an unprecedented outbreak in humans. Pigs are an important intermediate host for influenza; thus, we assessed the replication ability of three human H7N9 viruses (A/Anhui/1/2013, A/Shanghai/1/2013, A/Shanghai/2/2013) in swine tissue explants. All viruses tested replicated efficiently in explants from tracheas and bronchi, with limited replicati...

A method for culturing explants of lamellar hoof was developed to investigate the process of lamellar separation that occurs in laminitis. Explants, consisting of hoof wall, dermal and epidermal lamellae and the adjacent sub-lamellar connective tissue remained intact when cultured in tissue culture medium for 2 days. However, when cultured in the presence of the matrix metalloproteinase (MMP) a...

Perinatal (1–2 days old) and month-old (24–32 days old) male goats were used to investigate the effect of age and chronic culture (24 h) of perirenal and omental adipose tissue explants in the presence of insulin, cortisol and recombinant bovine somatotrophin (bGH), alone or in different combinations, on lipogenic responses in the absence and in the presence of catecholamines in acute incubatio...

Cardiomyocytes are principal cells in the heart. Earlier it was believed that cardiomyocytes cannot divide as these are terminally differentiated cells but latest research proved that these cells can divide and it has also been reported that there are also cardiac stem and progenitor cells in the heart. Cardiac stem cells and progenitor cells have a great potential for cellular therapy after my...

Circadian clocks impact vital cardiac parameters such as blood pressure and heart rate, and adverse cardiac events such as myocardial infarction and sudden cardiac death. In mammals, the central circadian pacemaker, located in the suprachiasmatic nucleus of the hypothalamus, synchronizes cellular circadian clocks in the heart and many other tissues throughout the body. Cardiac ventricle explant...