The LIPID MAPS consortium has developed a number of online tools for performing tasks such as drawing lipid structures and predicting possible structures from mass spectrometry (MS) data. A simple online interface has been developed to enable an end-user to rapidly generate a variety of lipid chemical structures, along with corresponding systematic names and ontological information. The structu...

Subcellular lipidomics is a novel field of research that requires the careful combination of several pre-analytical and analytical steps. To define a reliable strategy for mitochondrial lipid profiling, we performed a systematic comparison of different mitochondria isolation procedures by western blot analyses and comprehensive high-resolution lipidomics. Using liver-derived HepG2 cells, we com...

Background/Aims: The use of novel cryo-additive agents to increase cell viability post-cryopreservation is paramount improve future based-therapy treatments. We aimed establish the Human Leukemia (HL-60) cells lipidomic and biological patterns when cryo-preserved in DMSO alone with 300 µM Nigerose (Nig), 200 Salidroside (Sal) or a combination Nig (150 µM) Sal (100 µM). Methods: HL-60 were pre-i...

In a letter to the Editor of this Journal, it has been stated that cardiolipin Kolmer tests are consistently anticomplementary when plasma is used in place of serum (Klein, 1956). Since this test was not included in the paper criticized (Coleman, Appleman, and Kurtz, 1955), this report concerns our results using cardiolipin Kolmer antigen with serum and with plasma cleared of coagulated fibrino...

Oncocytic tumors are characterized by an excessive eosinophilic, granular cytoplasm due to aberrant accumulation of mitochondria. Mutations in mitochondrial DNA occur in oncocytic thyroid tumors, but there is no information about their lipid composition, which might reveal candidate theranostic molecules. Here, we used desorption electrospray ionization mass spectrometry (DESI-MS) to image and ...

The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80-90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the pres...