Equal volumes of plasma and 0.3 M K2HPO4, pH 7.4, were mixed, diluted 20-fold, and adjusted to pH 5.2. After incubation at 37#{176}C for 30 mm, the euglobulin precipitate, redissolved in 0.1 M K2HPO4, pH 7.4. developed caseinolytic activity (0.05 CTA U/mI). Na2HPO4 or NaCI of similar ionic strength could replace K2HPO4. The pH optimum of the protease was 6.5. activity falling off sharply below ...

Equal volumes of plasma and 0.3 M K2HPO4, pH 7.4, were mixed, diluted 20-fold, and adjusted to pH 5.2. After incubation at 37 degrees C for 30 min, the euglobulin percipitate, redissolved in 0.1 M K2HPO4, pH 7.4, developed caseinolytic activity (0.05 CTA U/ml). Na2HPO4 or NaCl of similar ionic strength could replace K2HPO4. The pH optimum of the protease was 6.5, activity falling off sharply be...

As to the change of factors relating to the plasmin system during pregnancy and puerperium, some conflicts have been found in the literature. This paper deals with the results of systemic analysis of this enzyme system, and the relation be tween sex hormones and the factors is discussed. A marked elevation of factors of plasmin system during pregnancy was observed in fibrinogen, spontaneous cas...

The tarnished plant bug, Lygus hesperus Knight, is a pest that causes considerable economic losses to vegetables, cotton, canola, and alfalfa. Detailed knowledge of its digestive physiology will provide new opportunities for a sustainable pest management approach to control this insect. Little is known about the different protease class contributions to the overall digestion of a specific prote...

Kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, Salinivibrio sp. strain MS-7, and maximum growth and protease activity was achieved after 48 hours at 30°C and 180 rpm. To determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and  maximum production of...

Degradation of extracellular matrix, particularly interstitial collagen, promotes plaque instability and contributes to restenosis after vascular injury. We have explored the effects of vitamins C and E on the collagen content and metalloproteinase-1 (MMP-1) expression after angioplasty in hypercholesterolemic pigs. Iliac angioplasty was performed on 18 minipigs divided into three diet groups: ...

The administration of doxycycline prior to bleomycin in mice attenuated pulmonary fibrosis. Bronchoalveolar neutrophil influx and gelatinase activity, but not caseinolytic activity, were attenuated by doxycycline. Established fibrosis was not affected by doxycycline. Thus, doxycycline might be useful for slowing down pulmonary fibrosis by biological activity other than antibacterial activity.

this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...

kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, salinivibrio sp. strain ms-7, and maximum growth and protease activity was achieved after 48 hours at 30°c and 180 rpm. to determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and  maximum production of...

In this study, Enterococcus faecalis proteolytic strains which have the potential to degradation of bovine milk proteins were isolated from Turkish White Pickled Cheeses and samples. E. found strong caseinolytic activity. The extracellular protease enzymes produced by 60 different samples analyzed in pattern bands on a stained SDS-PAGE gel. highest activity isolates determined at pH 7.0 40 ℃ fo...