This paper reports a RP-HPLC method for the determination of Lomustine (CCNU) in mouse tumor tissue. CCNU was separated and determined on a reverse-phase C18 5 microm column with a mobile phase of H2O-acetonitrile (54:46), detected at 254 nm and no internal standard. The calibration curve was linear (r = 0.9999) within the range of 0.98-15.68 microg/ml for CCNU. The recovery ratio of CCNU was 6...

-The effect of combinations of the conventional chemotherapeutic agent 1-(2-chloroethyl)-3-cyclohexyl-l-nitrosourea (CCNU) and nitroimidazole radiation sensitizers was evaluated in female C3H mice. Tumour response to single-agent or combination therapy was assessed in a tumour growth-delay assay. In the KHT sarcoma the simultaneous addition of misonidazole (MISO) was found to increase significa...

The antitumor activity of 1-(2-cMoroethyl)-3-cyclohexyl-1nitrosourea (CCNU) against the murine ependymoblastoma, glioma 26, and glioma 261 was compared with the uptake and distribution of the †̃ 4C-labeled drug in the murine ependymoblastoma. With a modified Wilcoxon rank-sum test, it was found that CCNU increased life-span in tumor animals from 2to 4-fold, depending on the dose used and the p...

Using the pZ189 shuttle vector approach, we determined two chloroethyl-cyclohexyl-nitrosourea (CCNU)-induced mutation spectra (3 and 6 mM) in African green monkey kidney cells (CV1). One hundred and twenty-one independent clones (101 CCNU induced, 45 at 3 mM and 56 at 6 mM; 20 spontaneous) showing functional inactivation of the supF gene were analyzed. One hundred and five plasmids (91 CCNU ind...

1,(2-Chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) and other chloroethylnitrosourea anticancer agents in clinical use produce severe and cumulative bone marrow toxicity. Chlorozotocin, a glucose analogue, has demonstrated reduced hematologic toxicity while retaining full antitumor activity. The biochemical-pharmacologic properties of chlorozotocin and CCNU were compared in human bone marrow. A...

Two lines of human colonic carcinoma cells have different sensitivities to 5-fluorouracil and 1-(2-chlorethyl)-3-(4-methylcyclohexyl)-1-nitrosourea (methyl-CCNU). The growth of the BE line is 50% inhibited by 330 microM 5-fluorouracil and 15 microM methyl-CCNU, and the HT-29 line is 50% inhibited by 85 and 140 microM, respectively. On cloning, 50% of BE cells are killed by 500 microM 5-fluorour...

Abstract Glioblastoma (GBM) is the most common primary malignant brain tumor in adults. Temozolomide (TMZ) part of standard care for patients with newly diagnosed GBM (ndGBM). Expression enzyme MGMT involved TMZ-induced DNA damage repair associated irresponsiveness some to TMZ. The addition lomustine (CCNU) TMZ demonstrated clinical benefit ndGBM. Tumor Treating Fields (TTFields) are electric f...

Abstract Background Temozolomide (TMZ) is the standard of care chemotherapy for newly diagnosed glioblastoma (ndGBM), most common primary malignant brain tumor in adults. However, 50% patients do not respond to TMZ due expression O6-methylguanine-DNA methyltransferase (MGMT), enzyme involved repair TMZ-induced damage. Tumor Treating Fields (TTFields) are alternating electric fields that display...

PURPOSE To evaluate long-term survival in a prospective series of patients newly diagnosed with glioblastoma and treated with a combination of lomustine (CCNU), temozolomide (TMZ), and radiotherapy. PATIENTS AND METHODS Thirty-nine patients received radiotherapy of the tumor site only (60 Gy) and CCNU/TMZ chemotherapy (n = 31 received standard-dose CCNU, 100 mg/m2 on day 1 and TMZ 100 mg/m(2)...