objective: development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. this study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with e1a 13s protein on human tissue plasminogen activator (t-pa) expression in chinese hamster ovary (ch...

enterohemorrhagic escherichia coli (ehec), such as e. coli o157:h7, are emerging food-borne pathogens worldwide. this micro-organism can damage the epithelial tissue of the large intestine. the cytotoxic effects can be neutralized by probiotics such as bifidobacterium bifidum. probiotics are viable cells that have beneficial effects on the health of the host. the preventing activity of b. bifid...

background: the aim of this study was to fabricate the poly caprolactone (pcl) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (hfsc) in the graft material using electrospun pcl nanofiber scaffold for tissue engineering applications. methods: the bulge region of rat whisker was isolated and cultured in dmem: n...

objective: tissue engineering is an (interdisciplinary field that applies polymeric scaffolds to control tissue formation in three-dinemtion (3d). the scaffold provides the microenvironment (synthetic temporary extracellular matrix) for regenerative cells, supporting cell attachment, proliferation, differentiation, and neo tissue genesis due to their suitable chemical, physical and biological s...

Functional graphenic material (FGM) scaffolds instruct bacterial attachment through electrostatic interactions with the cell wall.

The availability of synchronous cultures of untransformed CV-I cells has allowed analysis of the role of cell-substrate attachment in cell cycle progression as well as the ability of serum and SV40 infection to override the requirement for cell-substrate attachment. Attachment to a solid substrate is required for progression through G1. Prevention of attachment results in cell cycle arrest 30 m...

Enterohemorrhagic Escherichia coli (EHEC), such as E. coli O157:H7, are emerging food-borne pathogens worldwide. This micro-organism can damage the epithelial tissue of the large intestine. The cytotoxic effects can be neutralized by probiotics such as Bifidobacterium bifidum. Probiotics are viable cells that have beneficial effects on the health of the host. The preventing activity of B. bifid...

aim and background: complex nature of children`s affectionate problems requires assessment and usage of modern treatments. the present study aimed to investigate the effects of attachment-based therapy on depression symptoms in girl students of primary school who had attachment problems. methods and materials: this study was an empirical plan with pretest-posttest, follow up and control group. ...