Clinical studies have shown that in pathological conditions such as endometriosis and reproductive tract infection (male and female) there is an activation status of macrophages that produce large quantities of nitric oxide (NO) in addition to other effector molecules. Large amounts of NO may have embryotoxic roles and produce infertility. This study was designed to evaluate the effect of diffe...

clinical studies have shown that in pathological conditions such as endometriosis and reproductive tract infection (male and female) there is an activation status of macrophages that produce large quantities of nitric oxide (no) in addition to other effector molecules. large amounts of no may have embryotoxic roles and produce infertility. this study was designed to evaluate the effect of diffe...

There is great interest worldwide in discovering and developing a permanent source of tissues which would be capable of generating any cell type and which would avoid the problem of transplant rejection. Stem cells are cells that can specialize into the many different cells found in the human body. The ethical objections concerning stem cells have focused primarily on their source. Human embry...

Background: This study was carried out to evaluate the pattern of reactive oxygen species (ROS) variations during in vitro oocyte maturation and embryo development in sheep, and to investigate whether embryo culture condition and the presence of cell-permeable superoxide dismutase mimetic [Manganese (III) meso-tetrakis (4-benzoic acid) porphyrin (MnTBAP)] can influence ROS pattern. Materials an...

there is great interest worldwide in discovering and developing a permanent source of tissues which would be capable of generating any cell type and which would avoid the problem of transplant rejection. stem cells are cells that can specialize into the many different cells found in the human body. the ethical objections concerning stem cells have focused primarily on their source. human embryo...

most of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. the general use of nmri outbred mice is a model for toxicology, teratology and pharmacology. nmri mouse embryo exhibit the two-cell block in vitro. the objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygo...

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...

the effect of in planta tiba and proline on in vitro seedlings and cell culture of sugar beet was investigated. sterilized seeds were grown in vitro on ½ strength ms medium supplemented with 0 or 3 mg/l tiba. calli obtained on young leaves cultured on ms medium containing 1 mg/l bap, were used for the intiation of cell suspension cultures using ms basal composition supplemented with 0 or 50 m m...

introduction: mouse embryos can successfully be frozen with sucrose and propandiol. however, different developmental stages of mammalian embryos have shown to have various capacities to undergo freeze-thawing procedure. in the present study, the ability of 1,2,4 and 8-ell mouse embryo to tolerate the freeze-thawing procedure was investigated and the effect of this procedure on the further in vi...

introduction: it is now generally accepted that vero cells provide beneficial effects on improving embryo quality in many animal species. the aim of this research was to determine the effect of vero cell co-culture on the development of vitrified 8-cell mouse embryos. material and methods: eight- cell mouse embryos were provided from superovulated female nmri mice and divided into treatment cas...