Background Methods for haplotyping and DNA copynumber typing of single cells are paramount for studying genomic heterogeneity and enabling genetic diagnosis. Before analyzing the DNA of a single cell by microarray or next-generation sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts the frequency and composition of the cell’s alleles. As a conseque...

Background Methods for haplotyping and DNA copynumber typing of single cells are paramount for studying genomic heterogeneity and enabling genetic diagnosis. Before analyzing the DNA of a single cell by microarray or next-generation sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts the frequency and composition of the cell’s alleles. As a conseque...

plant growth is a result of cell division and cell enlargement. expansins are proteins that mediate long-term extension of isolated cell walls and they play a variety of roles in plant cells. in this study the atexpb2 gene was over-expressed in arabidopsis thaliana. the gene was isolated from genomics dna of arabidopsis and cloned into mediated vectors; pgemt and pbi121 vectors. transformation ...

Recent Technological Advances in cell-sorting and high throughput sequencing have enabled unprecedented insight into the cell at resolution of single cells further led to a massive accumulation omics data such as genomics, proteomics, epigenomics transcriptomics etc. In this article, we presented dissection single-cell gene expression, which turn reshapes large number transcriptomic studies. We...

Background: DNA amplification method has been developed for identifying and discriminating Salmonella serovars, using specific primers at the genus and serovar levels and to identify the S. Enteritidis, S. Dublin, S. Gallinarum and S. Pullorum. Objectives: This study was conducted for molecular identification and discrimination among some important Salmonella serovars. Methods: Fifty isolates o...