We report engineering Neurospora crassa to improve the yield of cellobiose and cellobionate from cellulose. A previously engineered strain of N. crassa (F5) with six of seven β-glucosidase (bgl) genes knocked out was shown to produce cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. In this study, the F5 strain was further modified to improve the ...

The oleaginous yeast Yarrowia lipolytica is an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known for decades, the unique native metabolism of Y. lipolytica for using complex fermentable sugars, which are abundant in lignocellulosic biomass, is poorly understood. In this study, we activated and ...

Four forms of cellobiose quinone dehydrogenase have been purified from the white-rot fungus Sporotrichum pulverulentum. The Mr of the enzyme has been estimated by sedimentation equilibrium to be 57,800 and by SDS/polyacrylamide-gel to be 60,000. These enzymes are clearly monomers. Cellobiose quinone dehydrogenases contain FAD and variable amounts of a green chromophore which we suggest is 6-hyd...

Cellobiose dehydrogenase (CDH) is capable of oxidizing cellobiose and related carbohydrates generating electrical current at carbon-based electrodes through direct electron transfer (DET) or mediated (MET) mechanisms. As a result, CDHs have been utilized as biocatalysts in biosensors biofuel cell anodes. A novel engineered ascomycetous Class II CDH with enhanced glucose activity was tested bioe...

Production of enzymes degrading plant cell walls was studied using media containing cellobiose or ammonium ions (NH,+) as limiting nutrients. Carboxymethylcellulase (CMcellulase), xylanase and pectin lyase were primarily cell-associated during exponential growth in batch culture but accumulated in the supernatant during the stationary phase. Activities of CM-cellulase and xylanase were higher i...