Nanocrystalline cellulose is an amphiphilic, high surface area material that can be easily functionalized and is biocompatible and eco-friendly. It has been used singularly and in combination with other nanomaterials to optimize biosensor design. The attachment of peptides and proteins to nanocrystalline cellulose and their proven retention of activity provide a route to bioactive conjugates us...

Actinomycetes is one of the microorganisms which have ability enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer hydrolase can be used a candidate probiotic. A probiotic should good resistance in different environmental conditions including pH salinity.  This research conducted to filter candidate’s probiotics from bacteria that tolerance salinity has activity ...

Studies were conducted on the Bacillus sp. neutral protease immobilization on different matrices. The immobilized protease was prepared by three methods, such as entrapment on calcium alginate and gelatin; physical adsorption by celite and alumina; ionic binding by amberlite IR-120 and DEAE-cellulose. The maximum immobilization yield was obtained with celite adsorbed protease. The immobilized e...

A 15-kDa antifungal protein, designated ganodermin, was isolated from the medical mushroom Ganoderma lucidum. The isolation procedure utilized chromatography on DEAE-cellulose, Affi-gel blue gel, CM-Sepharose and Superdex 75. Ganodermin was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and CM-Sepharose. Ganodermin inhibited the mycelial growth of Botrytis cinerea, Fusarium oxys...

The nano-Fe3O4 particles were prepared by hydrothermal method. Cellulose-based magnetic Fe3O4 was prepared by adding cellulose solution dissolved in sodium hydroxide / thiourea / urea system. The basic protease was used as the catalyst and glutaraldehyde was used as the crosslinking agent, The production of magnetic recyclable immobilized enzyme can greatly reduce the cost of hydrolysis of slud...

A procedure is described for the purification of a neutral protease from fermentation broths of Vibrio proteolyticus. The key feature of the purification scheme is the selective, irreversible inactivation of a contaminating exoenzyme, aminopeptidase, by alkali treatment, rather than removal of this enzyme by conventional chromatographic methods. Fermentation broths or concentrates were brought ...

the date palm fruit stalk borer is one of the most important pests of date palm in the world. biochemical properties of digestive proteases in oryctes elegans prell larvae were investigated in this research and optimal total proteolytic and trypsin activities were obtained at ph 9.0 and 11.0, respectively. activity staining of protease on sds-page showed one isoform. also, zymogram pattern of t...

Low salt extracts from homogenates of bovine cardiac muscle contain two protease inhibitors, one specific for the calcium-activated protease from this tissue and the other for trypsin and chymotrypsin, but no other serine proteases, including plasmin, thrombin, and subtilisin. The former, which can be separated from the protease by chromatography on DEAE-cellulose, is a protein with a molecular...

Cellulose-binding domains (CBDs) are protein domains with cellulose-binding activity, and some act as leaders in the localization of cellulosomal scaffoldin proteins to the hydrophobic surface of crystalline cellulose. In this study, we found that a CBD fusion enhanced and improved soluble β-glucuronidase (GusA) enzyme properties through the formation of an artificially oligomeric state. First,...

Ribosomes were isolated from E. coli, strain P, as previously described [S] . After extraction of ribosomal proteins by acetic acid protein S6 was separated from the other proteins by a two step elution procedure followed by a gradient elution on DEAE-cellulose. This resulted in several forms of S6 as described below and elsewhere in detail [4]. Amino acid analyses were done on a Durrum D-500 a...