LEVEL: INTERMEDIATE P roduction of monoclonal antibodies from mammalian cell culture has become ubiquitous in the biotechnology industry as companies continue to identify opportunities to treat diseases with such therapeutic proteins. The first step in recovery of secreted antibodies is to remove most insoluble components of the cell culture from the product stream. These components consist of ...

For traditional centrifugal capillary pressure curve measurements, a short core plug is often employed. The capillary pressure is assumed to be zero at the outflow boundary of the core. The traditional experiments are time consuming. Measurement of the full capillary pressure curve often requires 7 to10 different centrifuge speeds, thus requiring one day to several days for measurement. Differe...

Abstract This study compared a field versus reference laboratory technique for extracting (syringe vs. centrifuge) and analyzing sweat [Na(+)] and [K(+)] (compact Horiba B-722 and B-731, HORIBA vs. ion chromatography, HPLC) collected with regional absorbent patches during exercise in a hot-humid environment. Sweat samples were collected from seven anatomical sites on 30 athletes during 1-h cycl...

BACKGROUND Although platelet-rich plasma (PRP) is nowadays a common method in various medical fields, including cosmetic surgery or dermatology, the expensiveness of the kit for processing is still a hurdle. METHODS A new unique economic method for preparing PRP was reported. The method consists in a simple modification of a disposable 5-mL syringe that allows insertion into a common centrifu...

Internally cured (IC) concrete is frequently produced in North America using pre-wetted lightweight aggregate (LWA). One important aspect associated with the production of quality IC concrete is the accurate determination of the moisture content, including absorbed moisture and surface moisture of the LWA. Knowledge of the moisture content enables aggregate moisture corrections to be made for t...

BACKGROUND A simple and rapid slide Polybrene method (SP) for pretransfusion compatibility testing is described. SP is particularly suitable for use in developing countries where, due to limited resources, centrifuges and biologic reagents may not be readily available. STUDY DESIGN AND METHODS The original manual Polybrene method (MP) was modified for use on glass microscope slides, eliminati...

This report describes the development of a hand-powered centrifuge to determine hematocrit values in low-resource settings. A hand-powered centrifuge was constructed by using a salad spinner. Hematocrit values were measured by using the hand-powered device, and results were compared with those of a benchtop centrifuge. The packed cell volume (PCV) measured with the hand-powered device correlate...

Porous silicon (PSi) is an innovative inorganic material that has been recently developed for various drug delivery systems. For example, hydrophilic and hydrophobic PSi microparticles have been utilized to improve the dissolution rate of poorly soluble drugs and to sustain peptide delivery. Previously, the well-plate method has been demonstrated to be a suitable in vitro dissolution method for...

Purpose The purpose of this study was to evaluate the effect of gravity acceleration on choroidal and retinal nerve fiber layer (RNFL) thickness using swept-source optical coherence tomography (SS-OCT). Methods Thirteen healthy volunteers who planned to participate in human centrifuge training as part of the flight surgeon selection process enrolled this study. During centrifuge training, gra...