Aluminum is acutely toxic, and elevated concentrations of dissolved Al can have detrimental effects on both terrestrial and aquatic ecosystems. Robust analytical methods that can determine environmentally relevant Al fractions accurately and efficiently are required by the environmental monitoring community. A simple, robust passive sampling method, the diffusive gradients in thin films (DGT) t...

Sea-water samples collected by a variety of clean sampling techniques yielded consistent results for copper, cadmium, zinc, and nickel, which implies that representative, uncontaminated samples were obtained. A dithiocarbamate extraction method coupled with atomic absorption spectrometry and electro,thermal atomization is described which is essentially 100% quantitative for each of the four met...

The characteristics and location of rat intestinal proteins with calcium binding properties were reexamined using both a 45Ca-equilibrated Sephadex G-100 column and the chelex 100 method in the assay of 45Ca binding activity. The rat intestinal mucosa was found to have three different proteins with calcium binding properties. Two of these proteins were found to be vitamin D-dependent and were e...

3-Mercaptopropyl functionalized SBA-15 (SH-SBA) and 3-mercaptopropyl functionalized ethenylene bridged periodic mesoporous organosilica (SH-PMO) were included in a Diffusive Gradients in Thin film (DGT) probe and compared to similar commercially available resins also containing thiol functional groups, such as Sumichelate Q10R (SQR) and 3-mercaptopropyl functionalized silica gel (SH-KG), and al...

PCR-based analysis of skeletonized human remains is a common aspect in both forensic identification as well Ancient DNA research. In this, areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality recovered presence inhibitory substances samples from excavated remains. To enable amplification based remains, development optimized extractio...

This paper reports on the adsorption of Cr(VI), Cu(II) and Pb(II) from laboratory solution by the use of Chelex 100 (C100) and Amberlite IRA400 ion exchange resins. Metal loading at initial pHs of 3 to 8 at 1-100 mg/L were studied based on Q max and K f values of the Langmuir and Freundlich isotherm models, respectively. Lead was sorbed the most (Q max = 75.19 mg/g, at pH 6), followed by Cu (Q ...

AIM To provide a more efficient method for isolating DNA from peripheral blood for use in diagnostic DNA mutation analysis. METHODS The use of blood impregnated filter paper and Chelex-100 in DNA isolation was evaluated and compared with standard DNA isolation techniques. RESULTS In polymerase chain reaction (PCR) based assays of five point mutations, identical results were obtained with DN...

Four different DGT binding layers were used to make selective measurements of trace metals in coastal waters within The Broadwater (Gold Coast, Queensland). Chelex and PAMPAA (polyacrylamide-polyacrylic acid) binding layers were used to measure cations (Cd, Co, Cu, Mn, Ni, Pb, Zn), and Metsorb was used to measure anions (Al, As, Mo, Sb, V, W). A mixed binding layer (MBL) containing both Chelex ...