By means of a tissue culture technique, cells from chick embryos were procured in a state which proved to be suitable for electron microscopy. The electron micrographs disclosed details of cell structure not revealed by other methods of examination.

In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures...

This study investigated whether the same cytochrome P-450 (P-450) isoenzymes were inducible in cultures of chick-embryo hepatocytes as in the liver of chicken embryos. We purified two isoenzymes of cytochrome P-450 from the livers of 17-day-old-chick embryos: one of molecular mass approx. 50 kDa induced in vivo by the phenobarbital-like inducer glutethimide, and the second of approx. 57 kDa ind...

1. A method is described for the primary growth of chick embryo liver cells on cover slips in culture, and the factors of the culture medium are considered that affect the induction of porphyrin formation with certain chemicals. 2. A method is described for following &aminolevulinic acid synthetase (ALA synthetase) activity by the determination of porphyrin fluorescence developed in the cells o...

The study aimed to investigate the effect of in ovo (IO) leptin injection on yolk sac concentration, embryo development, blood lipid metabolism, and biochemical parameters broiler chicks during early post-hatching period. Hatching eggs were weighed placed an incubator with 100 eggs/4 replications/group. groups were: non-injected group (C); 3 injected μl phosphate buffer solution (PBS); 0.5 (L0....

Of 129 strains of the Hong Kong variant of influenza A2 virus isolated from 176 respiratory infections, 105 (81.4%) were isolated in tissue culture, the remainder being detected only by the inoculation of fertile hen's eggs. Organ cultures of chick embryo trachea were at least as sensitive as monkey kidney tissue culture for the isolation of this virus but organ cultures of human embryonic cili...

Uridine diphosphate (UDP) glucuronyltransferase activity in chick liver rises at hatching from near zero to adult levels. This rise will occur prematurely in embryo liver during organ culture. Increase in enzyme activity during organ culture differs with embryo age: in liver from 11-day old embryos it ceases at adult values; in liver from 5-day old embryos it continues to much higher-than-adult...

Rabies virus has been propagated in serum-Tyrode solution containing either embryo mouse brain or embryo chick brain. The culture virus reached a titre of 3 x 10(-5) cc. after 4 days' incubation at 37 degrees C., and survived at least 2 months at 5 degrees C. in the liquid or dry state.