The monoclonal antibody 27/21 directed against mouse nerve growth factor (NGF) interferes in vivo with the survival of sensory dorsal root ganglion (DRG) neurones during the development of the quail embryo: the number of DRG neurones at embryonic day 11 (E11) was reduced by about 30% in embryos treated with the antibody between E3 and E11. Neurone numbers in the nodose ganglion were not affecte...

Chick-mouse heterokaryons were obtained by UV-Sendai virus-induced fusion of chick erythrocytes with thymidine (dT) kinase-deficient mouse fibroblast [LM(TK(-))] cells. Autoradiographic studies demonstrated that 1 day after fusion, [(3)H]dT was incorporated into both red blood cell and LM(TK(-)) nuclei of 23% of the heterokaryons. Self-fused LM(TK(-)) cells failed to incorporate [(3)H]dT into n...

The study aimed to investigate the effect of in ovo (IO) leptin injection on yolk sac concentration, embryo development, blood lipid metabolism, and biochemical parameters broiler chicks during early post-hatching period. Hatching eggs were weighed placed an incubator with 100 eggs/4 replications/group. groups were: non-injected group (C); 3 injected μl phosphate buffer solution (PBS); 0.5 (L0....

The tridecamer d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to 13 nucleotides of the 3'- and 5'-reiterated terminal sequences of Rous sarcoma virus 35S RNA, was added to chick embryo fibroblast tissue cultures infected with Rous sarcoma virus. Inhibition of virus production resulted. The inference emerges that the tridecamer and its counterpart with blocked 3'- and 5'-hydroxyl termini e...

The effects of chloroquine on the growth and morphology of fibroblasts in tissue culture, and in vivo granulomas were investigated. As the result, the drug was shown to have a potent action to inhibit fibroblast growth, which has led to a possibility of its clinical use to patients with malignant tumors. ∗PMID: 14165348 [PubMed OLDMEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Med...

the purpose of this experimental study was to evaluate the effect of chicken amniotic fluid (af) on a cross section of rat sciatic nerves. thirty adult male sprague-dawley rats weighing 275 to 300 g, were randomized into three groups treated with (1) amniotic fluid or af (n=10), (2) normal saline or ns (n=10), and (3) sham surgery (n=10). the af was aspirated from the amniotic cavity of incubat...

introduction: there is considerable evidence that glutamate-mediated excitatory neurotransmission plays an important role in mediating the behavioral actions of acutely administered ethanol. the aim of the present study was to investigate the effect of acute ethanol exposure on nr1 subunit of nmda (n-methyl-d-aspartate) receptor distribution in the cerebral cortex of chick embryo on the 10th an...

A chick-embryo fibroblast lambda gt11 cDNA library was screened with affinity-purified antibodies to chick gizzard vinculin. One recombinant was purified to homogeneity and the fusion protein expressed in Escherichia coli strain C600. The fusion protein was unstable, but polypeptides that reacted with vinculin antibodies, but not non-immune immunoglobulin, were detected by Western blotting. The...