Objective: The aim of this study was to compare four different laboratory methods for detection of methicillin resistant Staphylococcus aureus (MRSA). Design: The clinical specimens including urine, blood, wound and tracheal tube aspirates were processed for isolation of S .aureus. Setting: The samples were obtained from patients admitted in Milad hospital in Tehran,Iran. Subjects: 95 strains o...

The MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was blindly compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, and PCR of the mecA gene for the detection of methicillin resistance in Staphylococcus aureus. A total of 120 methicillin-susceptible S. aureus (MSSA) ...

Background and Aims: The aim of present study was to investigate the molecular characteristic of Staphylococcus aureus to detect mec A gene and to type SCCmec in strain isolated from healthy worker at Larestan Hospital. Material and Methods: This study was carried out from the 250 nasal swab healthy worker  at Larestan hospital. Multiplex polymerase chain reaction for mecA gene was perf...

Active surveillance for methicillin-resistant Staphylococcus aureus (MRSA) is among the strategies recommended by the Society for Healthcare Epidemiology of America for control of nosocomial MRSA infections. Infection control and laboratory personnel desire rapid, sensitive, and inexpensive methods to enhance surveillance activities. A multicenter study was performed to evaluate a new selective...

In this study, we report the antibacterial activities of six polyphenols (i.e., luteolin, quercetin, scutellarin, apigenin, chlorogenic acid, and resveratrol) against 29 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA), and in vitro antibacterial activities of two-drug combinations. All of the MRSA strains evaluated were clinical isolates from patients with MRSA bacteremi...

Sir, We have with interest read the recent article by Paterson et al. describing the prevalence of mecC-carrying methicillin-resistant Staphylococcus aureus (MRSA) in England. In that study, the prevalence of mecC MRSA was 0.45% of all MRSA. In contrast, in a recent study mecC-positive MRSA constituted 2.8% of all MRSA in Denmark in 2011. This difference may be caused by a different epidemiolog...

The emergence of Methicillin-resistant Staphylococcus aureus (MRSA) has posed a serious therapeutic challenge. We report the prevalence and antibiotic susceptibility pattern of MRSA in the hospitalized patients in a rural tertiary care hospital in India. The study comprised of 97 Staphylococcus aureus isolated from a total of 400 clinical samples obtained from hospitalized patients. Antibiotic ...

Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...

Infections caused by Methicillin-resistant Staphylococcus aureus (MRSA) is a growing problem. Medical students are at risk of getting infected by MRSA during their training due to exposure with infected patients and may be a source of transmission for other patients. Therefore it may be important to know nasal carriage of MRSA among medical students going for clinical posting. This prospective ...

Rapid laboratory diagnosis is critical for treating, managing, and preventing methicillin-resistant Staphylococcus aureus (MRSA) infections. We evaluated and compared the potential for MRSA detection of five chromogenic media, Brilliance MRSA agar (Oxoid), ChromID (bioMérieux), MRSASelect (Bio-Rad), CHROMagar (CHROMagar Microbiology), and BBL-CHROMagar (BD Diagnostics). Media were tested with l...