background: evaluating the significance and the effects of plant-derived drugs on laboratory animal’s fertility was recognized. there was antioxidant activity reported from heracleum persicum (golpar).objective: current study aims to study the antioxidant effect of golpar extracts on sperm parameters and chromatin quality in mice.materials and methods: eighteen adult male mice were divided to 3...

Background: Varicocele is commonly existed among infertile men and causes testis damage. Disorder of spermatogenesis was observed in varicocele individuals and leads to low quality sperm parameters and chromatin damage. Chromatin damage plays a critical role in pregnancy outcomes through in vitro or in vivo fertilization. Varicocelectomy considered as standard treatment of clinical varicocele. ...

background: the particles in the range of 1-100 nm are called nanoparticles56t. gold nanoparticle is one of the most important metal nanoparticles with wide usage56t.objective: 56tthis study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice56t.materials and methods: 56tin this experimental study, 72 male bulb-c mice were divided into 9 groups inc...

background: acrylamide (aa) is an important industrial chemical primarily. aa is also found in carbohydrate-rich foods that are prepared at high temperatures, such as french fries and potato chips. it is demonstrated that aa is a carcinogen and reproductive toxin and has ability to induce sperm damage. objective: the aim of this study was to observe the effects of aa on sperm parameters and eva...

Background: Acrylamid (AA) is found in carbohydraterich foods that are prepared at high temperatures, such as French fries and potato chips. The toxic effects of AA in male animals include degeneration of epithelial cells of seminiferous tubules, decreased number of sperm and elevation in abnormal spermatozoa. The aim of this study was to evaluate the detrimental effects of AA on sperm paramete...

in this study the effect of a methylated derivative of nitrogen mustard (mechloroethamin) on solubilized and reconstituted chromatin was investigated using uvnis spectroscopy, dna and sds gel electrophoresis and densitomeoic scanning techniques, the results indicated that the 210 and 260 nm absorbances of reconstituted chromatin were decreased in a dose-dependent manner after treatment with var...

background: currently, relation between reactive oxygen species (ros) ros concentration and semen quality was indicated. saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. objective: the aim of this study was to evaluate the protection potency of saffron and vitamin e on sperm chromatin integrity. material...

BACKGROUND Sperm cells extracted from testes (TESE) have poor chromatin quality and motility. Various substances are used in the laboratory to increase sperm motility and improve the ART outcomes; however, there are few research which considered improving both sperm motility and chromatin quality. OBJECTIVE The aim of this investigation was to evaluate the improvement of the testicular sperm ...

in this study calf thymus chromatin was fractionated into active (s1 and s2) and inactive (p 2) chromatin. then the interaction of an anthracyc1ine antibiotic, adriamycin, with these fractions was investigated employing absorption and difference uv nis spectroscopy and sds and agarose gel electrophoreses. the results suggest that the binding of adriamycin to the s 1 fraction is slight but s2 an...

Chromatin shearing is the first critical step to provide sensitive and reproducible results in chromatin immunoprecipitation (ChIP) experiments. Adaptive Focused Acoustics (AFA) acoustic technology provides precise control over mechanical shearing and thermal control during processing to deliver high quality chromatin for the most sensitive results, in a highly reproducible manner. Optimizing t...