objective(s) a sensitive liquid chromatographic method for the analysis of clarithromycin- a macrolide antibiotic- in human serum, using pre-column derivatization with 9-fluorenylmethyl chloroformate (fmoc-cl) is described. materials and methods the method involved liquid-liquid extraction of the drug and an internal standard (amantadine) followed by pre-column derivatization of the analytes wi...

An Agilent ZORBAX sub-2 μm Rapid Resolution High Definition (RRHD) 300SB-C8 reversed-phase column was used under optimized chromatographic conditions for delivering highly resolved ultra fast separations of reduced and alkylated monoclonal antibodies. The StableBond C8 phase and RRHD column technology, in combination with robust gradient elution conditions enabled efficient, fast, and effective...

In this fully automated technique for sample cleanup before chromatographic or other quantitation steps, analytes in body fluids are enriched and semi-purified on a first column. After their selective elution, analytes are "transformed" by admixing appropriate solvents in such a way that they are focused on the top of a second column. By backflush, they then are transferred to an analytical liq...

I describe deactivation treatment of the OV-17 chromatographic column to minimize adsorption of tricyclic antidepressant drugs on the solid support of the column. The procedure involves heat treatment at 399 degrees C under a low flow of nitrogen, with bleeding of OV-17 liquid phase from the injector tube into the column. The column is then conditioned with vapors of phenyldiethanolamine succin...

Hydrophilic interaction chromatography (HILIC) was employed to investigate chromatographic behavior of selected flavonoids from their different subgroups differing in polarity. Chromatographic measurements were performed on two different HILIC columns: unmodified silica (Atlantis-HILIC) and zwitterionic sulfoalkylbetaine (SeQuant ZIC-HILIC). Separation parameters such as content and type of org...

In order to investigate the gas separation ability of a column packed with nanozeolitic material, nano NaY zeolite was synthesized and granulated. These uniform granules packed in a chromatographic column were utilized for separation of Xe and Kr under various operating conditions. With regards to the response peaks obtained from trace injections of Xe and Kr into the column, the first and seco...

A method of calculating the second dimension hold-up time for comprehensive two-dimensional gas chromatographic (GC×GC) data was developed by incorporating the temperature information of the second dimension column into the calculation model. The model was developed by investigating the relationship between the coefficients in each of six literature reported nonlinear models and the relationshi...

The selection of column packing during the development of high-performance liquid chromatography method is a crucial step to achieve sufficient chromatographic resolution of analyzed species in complex mixtures. Various stationary phases are tested in this paper for the analysis of complex mixture of triacylglycerols (TGs) in blackcurrant oil using non-aqueous reversed-phase (NARP) system with ...

We developed a high-performance liquid-chromatographic separation of five steroids (estriol, estradiol, cortisol, progesterone, and testosterone), eluting with a water-acetonitrile gradient from a reversed-phase (C18) column. By applying a simplex search algorithm to maximize a chromatographic-response function, we sought to optimize the original conditions of the chromatographic analysis, whic...